MARC details
000 -LEADER |
fixed length control field |
03127nab a22004457a 4500 |
001 - CONTROL NUMBER |
control field |
G95372 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
MX-TxCIM |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20221118224145.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
221118s2011 xxu|||p|op||| 00| 0 eng d |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
International Standard Serial Number |
1932-6203 (Online) |
024 8# - OTHER STANDARD IDENTIFIER |
Standard number or code |
https://doi.org/10.1371/journal.pone.0024699 |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
MX-TxCIM |
041 ## - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-6360 |
100 0# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Lin Li |
9 (RLIN) |
22830 |
245 10 - TITLE STATEMENT |
Title |
An 11-bp insertion in Zea mays fatb reduces the palmitic acid content of fatty acids in maize grain |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Place of publication, distribution, etc. |
San Francisco, CA (USA) : |
Name of publisher, distributor, etc. |
Public Library of Science, |
Date of publication, distribution, etc. |
2011. |
500 ## - GENERAL NOTE |
General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1932-6203 |
500 ## - GENERAL NOTE |
General note |
Peer review |
500 ## - GENERAL NOTE |
General note |
Open Access |
520 ## - SUMMARY, ETC. |
Summary, etc. |
The ratio of saturated to unsaturated fatty acids in maize kernels strongly impacts human and livestock health, but is a complex trait that is difficult to select based on phenotype. Map-based cloning of quantitative trait loci (QTL) is a powerful but time-consuming method for the dissection of complex traits. Here, we combine linkage and association analyses to fine map QTL-Pal9, a QTL influencing levels of palmitic acid, an important class of saturated fatty acid. QTL-Pal9 was mapped to a 90-kb region, in which we identified a candidate gene, Zea mays fatb (Zmfatb), which encodes acyl-ACP thioesterase. An 11-bp insertion in the last exon of Zmfatb decreases palmitic acid content and concentration, leading to an optimization of the ratio of saturated to unsaturated fatty acids while having no effect on total oil content. We used three-dimensional structure analysis to explain the functional mechanism of the ZmFATB protein and confirmed the proposed model in vitro and in vivo. We measured the genetic effect of the functional site in 15 different genetic backgrounds and found a maximum change of 4.57 mg/g palmitic acid content, which accounts for ~20?60% of the variation in the ratio of saturated to unsaturated fatty acids. A PCR-based marker for QTL-Pal9 was developed for marker-assisted selection of nutritionally healthier maize lines. The method presented here provides a new, efficient way to clone QTL, and the cloned palmitic acid QTL sheds lights on the genetic mechanism of oil biosynthesis and targeted maize molecular breeding. |
546 ## - LANGUAGE NOTE |
Language note |
Text in English |
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Maize |
Source of heading or term |
AGROVOC |
9 (RLIN) |
1173 |
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
9 (RLIN) |
9144 |
Topical term or geographic name as entry element |
Palmitic Acid |
Source of heading or term |
AGROVOC |
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
9 (RLIN) |
2084 |
Topical term or geographic name as entry element |
Chromosome mapping |
Source of heading or term |
AGROVOC |
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
9 (RLIN) |
8903 |
Topical term or geographic name as entry element |
Chromosome manipulation |
Source of heading or term |
AGROVOC |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Hui Li |
9 (RLIN) |
18792 |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Qing Li |
9 (RLIN) |
17870 |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
XiaoHong Yang |
9 (RLIN) |
19531 |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Debo Zheng |
9 (RLIN) |
29119 |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Warburton, M.L. |
9 (RLIN) |
4138 |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Yuchao Chai |
9 (RLIN) |
21871 |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Pan Zhang |
9 (RLIN) |
29120 |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Yuqiu Guo |
9 (RLIN) |
24858 |
700 1# - ADDED ENTRY--PERSONAL NAME |
9 (RLIN) |
398 |
Personal name |
Jianbing Yan |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
JianSheng Li |
9 (RLIN) |
19532 |
773 0# - HOST ITEM ENTRY |
Title |
PLoS ONE |
Related parts |
v. 6, no. 9, e24699 |
Place, publisher, and date of publication |
San Francisco, CA (USA) : Public Library of Science, 2011. |
Record control number |
G94957 |
International Standard Serial Number |
1932-6203 |
856 4# - ELECTRONIC LOCATION AND ACCESS |
Link text |
Open Access through DSpace |
Uniform Resource Identifier |
http://hdl.handle.net/10883/1853 |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Koha item type |
Article |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Suppress in OPAC |
No |