Knowledge Center Catalog

An 11-bp insertion in Zea mays fatb reduces the palmitic acid content of fatty acids in maize grain (Record no. 28595)

MARC details
000 -LEADER
fixed length control field 03127nab a22004457a 4500
001 - CONTROL NUMBER
control field G95372
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20221118224145.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 221118s2011 xxu|||p|op||| 00| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1932-6203 (Online)
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1371/journal.pone.0024699
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-6360
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Lin Li
9 (RLIN) 22830
245 10 - TITLE STATEMENT
Title An 11-bp insertion in Zea mays fatb reduces the palmitic acid content of fatty acids in maize grain
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. San Francisco, CA (USA) :
Name of publisher, distributor, etc. Public Library of Science,
Date of publication, distribution, etc. 2011.
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1932-6203
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Open Access
520 ## - SUMMARY, ETC.
Summary, etc. The ratio of saturated to unsaturated fatty acids in maize kernels strongly impacts human and livestock health, but is a complex trait that is difficult to select based on phenotype. Map-based cloning of quantitative trait loci (QTL) is a powerful but time-consuming method for the dissection of complex traits. Here, we combine linkage and association analyses to fine map QTL-Pal9, a QTL influencing levels of palmitic acid, an important class of saturated fatty acid. QTL-Pal9 was mapped to a 90-kb region, in which we identified a candidate gene, Zea mays fatb (Zmfatb), which encodes acyl-ACP thioesterase. An 11-bp insertion in the last exon of Zmfatb decreases palmitic acid content and concentration, leading to an optimization of the ratio of saturated to unsaturated fatty acids while having no effect on total oil content. We used three-dimensional structure analysis to explain the functional mechanism of the ZmFATB protein and confirmed the proposed model in vitro and in vivo. We measured the genetic effect of the functional site in 15 different genetic backgrounds and found a maximum change of 4.57 mg/g palmitic acid content, which accounts for ~20?60% of the variation in the ratio of saturated to unsaturated fatty acids. A PCR-based marker for QTL-Pal9 was developed for marker-assisted selection of nutritionally healthier maize lines. The method presented here provides a new, efficient way to clone QTL, and the cloned palmitic acid QTL sheds lights on the genetic mechanism of oil biosynthesis and targeted maize molecular breeding.
546 ## - LANGUAGE NOTE
Language note Text in English
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Maize
Source of heading or term AGROVOC
9 (RLIN) 1173
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 9144
Topical term or geographic name as entry element Palmitic Acid
Source of heading or term AGROVOC
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 2084
Topical term or geographic name as entry element Chromosome mapping
Source of heading or term AGROVOC
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 8903
Topical term or geographic name as entry element Chromosome manipulation
Source of heading or term AGROVOC
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Hui Li
9 (RLIN) 18792
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Qing Li
9 (RLIN) 17870
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name XiaoHong Yang
9 (RLIN) 19531
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Debo Zheng
9 (RLIN) 29119
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Warburton, M.L.
9 (RLIN) 4138
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Yuchao Chai
9 (RLIN) 21871
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Pan Zhang
9 (RLIN) 29120
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Yuqiu Guo
9 (RLIN) 24858
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 398
Personal name Jianbing Yan
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name JianSheng Li
9 (RLIN) 19532
773 0# - HOST ITEM ENTRY
Title PLoS ONE
Related parts v. 6, no. 9, e24699
Place, publisher, and date of publication San Francisco, CA (USA) : Public Library of Science, 2011.
Record control number G94957
International Standard Serial Number 1932-6203
856 4# - ELECTRONIC LOCATION AND ACCESS
Link text Open Access through DSpace
Uniform Resource Identifier http://hdl.handle.net/10883/1853
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme Dewey Decimal Classification
Suppress in OPAC No
Holdings
Date last seen Total Checkouts Full call number Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/05/2017   CIS-6360 07/05/2017 Article Not Lost Dewey Decimal Classification     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/05/2017

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