Knowledge Center Catalog

A one-tube fluorescent assay for the quarantine detection and identification of Tilletia indica and other grass bunts in wheat (Record no. 28494)

MARC details
000 -LEADER
fixed length control field 02376nab a22003137a 4500
001 - CONTROL NUMBER
control field G95217
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20171220113530.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1448-6032 (Revista en electrónico)
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0815-3191
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1071/AP08088
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title En
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Mui-Keng Tan
245 00 - TITLE STATEMENT
Title A one-tube fluorescent assay for the quarantine detection and identification of Tilletia indica and other grass bunts in wheat
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2009
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0815-3191
520 ## - SUMMARY, ETC.
Summary, etc. A molecular assay with enhanced specificity and sensitivity has been developed to assist in the surveillance of Karnal bunt, a quarantineable disease with a significant impact on international trade. The protocol involves the release of DNA from spores, PCR amplification to enrich Tilletia-specific templates from released DNA and a five-plex, real-time PCR assay to detect, identify and distinguish T. indica and other Tilletia species (T. walkeri, T. ehrhartae, T. horrida and a group comprising T. caries, T. laevis, T. contraversa, T. bromi and T. fusca) in wheat grains. This fluorescent molecular tool has a detection sensitivity of one spore and thus bypasses the germination step, which in the current protocol is required for confirmation when only a few spores have been found in grain samples. The assay contains five dual-labelled, species-specific probes and associated species-specific primer pairs in a PCR mix in one tube. The different amplification products are detected simultaneously by five different fluorescence spectra. This specific and sensitive assay with reduced labour and reagent requirements makes it an effective and economically sustainable tool to be used in a Karnal bunt surveillance program. This protocol will also be valuable for the identification of some contaminant Tilletia sp. in wheat grains.
546 ## - LANGUAGE NOTE
Language note English
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Ghalayini, A.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Jianping Yi,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Priest, M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sharma, I.,
Relator term coaut.
9 (RLIN) 1884
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Shivas, R.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Wright, D.,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Australasian Plant Pathology
Related parts v. 38, no. 2, p. 101-109
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/05/2017   07/05/2017 Article Not Lost     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/05/2017

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