Knowledge Center Catalog

Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.) (Record no. 27969)

MARC details
000 -LEADER
fixed length control field 02664nab a22004097a 4500
001 - CONTROL NUMBER
control field G93799
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20230811185610.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210706s2010 xxu|||p|op||| 00| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0733-5210
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1016/j.jcs.2010.01.005
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-5882
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Lin-hai Wang
9 (RLIN) 20836
245 10 - TITLE STATEMENT
Title Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. USA :
Name of publisher, distributor, etc. Elsevier,
Date of publication, distribution, etc. 2010.
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210
520 ## - SUMMARY, ETC.
Summary, etc. Low-molecular-weight glutenin subunits (LMW-GS) play a key role in determining the processing quality of the end-use products of common wheat. The objectives of this study were to identify genes at Glu-A3 locus, develop the STS markers, and establish multiplex PCR with the STS markers for Glu-A3 alleles. Gene-specific PCR primers were designed to amplify six near-isogenic lines (NILs) and Glenlea with different Glu-A3 alleles (a, b, c, d, e, f and g) defined by the protein electrophoretic mobility. Three Glu-A3 genes with complete coding sequence were cloned, designated as GluA3-1, GluA3-2 and GluA3-3, respectively. Seven dominant allele-specific STS (sequence tagged sites) markers were designed based on the SNPs (single nucleotide polymorphisms) among different allelic variants for the discrimination of the Glu-A3 protein alleles a, b, c, d, e, f and g. Four multiplex PCRs were established including Glu-A3b + Glu-A3f, Glu-A3d + Glu-A3f, Glu-A3d + Glu-A3g, and Glu-A3b + Glu-A3e. These markers and multiplex-PCR systems were validated on 141 CIMMYT wheat varieties and advanced lines with different Glu-A3 alleles, confirming that they can be efficiently used in marker-assisted breeding.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note Text in English
591 ## - CATALOGING NOTES
Affiliation Elsevier
594 ## - STAFFID
StaffID INT2411|INT0368
595 ## - COLLECTION
Collection CIMMYT Staff Publications Collection
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Wheat
Source of heading or term AGROVOC
9 (RLIN) 1310
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Glutenins
Source of heading or term AGROVOC
9 (RLIN) 1137
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Genetic markers
Source of heading or term AGROVOC
9 (RLIN) 1848
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element PCR
Source of heading or term AGROVOC
9 (RLIN) 12563
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Genying Li
9 (RLIN) 5949
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Peña-Bautista, R.J.
Field link and sequence number INT0368
Miscellaneous information Global Wheat Program
9 (RLIN) 645
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Xianchun Xia
9 (RLIN) 377
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name He Zhonghu
Miscellaneous information Global Wheat Program
Field link and sequence number INT2411
9 (RLIN) 838
773 0# - HOST ITEM ENTRY
Title Journal of Cereal Science
Related parts v. 51, no. 3, p. 305-312
Place, publisher, and date of publication USA : Elsevier, 2010.
Record control number G444514
International Standard Serial Number 0733-5210
856 4# - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier https://hdl.handle.net/20.500.12665/583
Link text Access only for CIMMYT Staff
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme Dewey Decimal Classification
Suppress in OPAC No
Holdings
Date last seen Total Checkouts Full call number Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/03/2017   CIS-5882 07/03/2017 Article Not Lost     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/03/2017

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