MARC details
| 000 -LEADER |
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| fixed length control field |
03352nab a22004697a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G93625 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20220512173943.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
220512s2008 xxc|||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0008-4220 |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1918-1833 (Online) |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
https://doi.org/10.4141/CJPS07013 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-5760 |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Aili Wang |
| 9 (RLIN) |
20799 |
| 245 10 - TITLE STATEMENT |
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| Title |
Rapid separation and characterization of grain water-soluble proteins in bread wheat cultivars (Triticum aestivum L.) by capillary electrophoresis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Ontario (Canada) : |
| Name of publisher, distributor, etc. |
Canadian Science Publishing, |
| Date of publication, distribution, etc. |
2008. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0008-4220 |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Water-soluble (WS) proteins in wheat grain are considered to represent the suite of biologically active enzymes and enzyme inhibitors in the grain. In this study, a rapid capillary electrophoresis (CE) method for WS protein separations was developed using untreated fused-silica columns and an acidic phosphate-glycine buffer system. In order to optimize the resolution and reproducibility of CE separation, different protein extraction methods, organic modifiers in phosphate-glycine buffer and capillary electrophoresis conditions, including capillary length and inner diameter (ID), operating temperature, performance voltages, sample injection times, etc., were investigated. High resolution and reproducibility of WS proteins were achieved using 20% ethanol as the extracting buffer. The optimal condition to separate these proteins was 50 μm ID × 31.5 cm (26.5 cm to the detector) capillary at 11.0 kV and 35°C. The optimum buffer was 0.1 M phosphate-glycine (pH 2.5) containing 20% acetonitrile (ACN) and 0.05% hydroxylpropylmethylcellulose. Using this method, the WS proteins were well separated in less than 10 min. A total of 120 Chinese bread wheat cultivars were analyzed. The CE patterns of most bread wheat cultivars showed a higher level of polymorphisms compared with SDS-PAGE patterns. All cultivars analyzed could be readily differentiated based on their WS protein profiles. Results indicate that the WS proteins are useful biochemical markers for wheat genetics and breeding research and CE is expected to become a new and powerful tool for the separation and characterization of grain WS proteins in bread wheat. |
| 536 ## - FUNDING INFORMATION NOTE |
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| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
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| Language note |
Text in English |
| 594 ## - STAFFID |
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| StaffID |
INT2411 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
1296 |
| Topical term or geographic name as entry element |
Triticum aestivum |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1265 |
| Topical term or geographic name as entry element |
Soft wheat |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1848 |
| Topical term or geographic name as entry element |
Genetic markers |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Wheat |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1310 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yuhe Pei |
| 9 (RLIN) |
20837 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xiaohui Li |
| 9 (RLIN) |
18575 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yanzhen Zhang |
| 9 (RLIN) |
24260 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Qiang Zhang |
| 9 (RLIN) |
6993 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
He Zhonghu |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2411 |
| 9 (RLIN) |
838 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xianchun Xia |
| 9 (RLIN) |
377 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Appels, R. |
| 9 (RLIN) |
1980 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wujun Ma |
| 9 (RLIN) |
1979 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xiu-Qiang Huang |
| 9 (RLIN) |
27409 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yueming Yan |
| 9 (RLIN) |
15752 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Canadian Journal of Plant Science |
| Related parts |
v. 88, no. 4, p. 843-848 |
| Place, publisher, and date of publication |
Ontario (Canada) : Canadian Science Publishing, 2008. |
| Record control number |
G444210 |
| International Standard Serial Number |
0008-4220 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
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| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/21413 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |