MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02838nab a22004217a 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
G93445 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20230804205719.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
210804t2009 xxk|||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1439-0523 (Online) |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
0179-9541 |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1111/j.1439-0523.2008.01618.x |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-5626 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xu, X. |
| 9 (RLIN) |
21840 |
| 245 12 - TITLE STATEMENT |
|---|
| Title |
A high-throughput, low cost gel-based SNP assay for positional cloning and marker assisted breeding of useful genes in cereals |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
United Kingdom : |
| Name of publisher, distributor, etc. |
Wiley, |
| Date of publication, distribution, etc. |
2009. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0179-9541 |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Several SNP (single nucleotide polymorphism) genotyping methods have been developed in the past most of which require sophisticated instrumentation and large initial investments. We describe here a high-throughput SSCP (single strand conformation polymorphism) system on our HEGS (high efficiency genome scanning) platform, which is simple, accurate, cost effective and requires neither restriction digestion of the amplification products nor elaborate post-PCR processing detection. Several parameters critical to SSCP analysis were optimized viz., gel matrix and concentration, gel running temperature, buffer composition, running conditions and PCR primer design so as to identify SNPs in amplicons ranging from 100 to 750 bp in size. A simple post-PCR processing system was developed using fluorescent dye for quick and easy detection of SNP polymorphism. HEGS-SSCP was also found to be useful in uncovering simple sequence repeat differences between different genotypes that differ by one or few di/tri nucleotide repeats. The practical utility of this system is illustrated with two successful efforts towards construction of high resolution linkage maps of a lesion mimic locus on chromosome 7 and a major quantitative trait locus conditioning field blast resistance on chromosome 4 in rice. |
| 536 ## - FUNDING INFORMATION NOTE |
|---|
| Text of note |
Global Maize Program |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
John Wiley |
| 594 ## - STAFFID |
|---|
| StaffID |
INT2925 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Single nucleotide polymorphisms |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
10805 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
PCR |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
12563 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genomics |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1132 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Marker-assisted selection |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
10737 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genetic markers |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1848 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cereals |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1036 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
875 |
| Personal name |
BABU, R. |
| Miscellaneous information |
Global Maize Program |
| Field link and sequence number |
INT2925 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
21841 |
| Personal name |
Fujimura, T. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
4170 |
| Personal name |
Shinji Kawasaki |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Plant Breeding |
| Related parts |
v. 128, no. 4, p. 325-331 |
| Place, publisher, and date of publication |
United Kingdom : Wiley, 2009. |
| Record control number |
G445212 |
| International Standard Serial Number |
1439-0523 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Access only for CIMMYT Staff |
| Uniform Resource Identifier |
https://hdl.handle.net/20.500.12665/337 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |