MARC details
| 000 -LEADER |
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| fixed length control field |
04321nab a22004457a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G91148 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20220523215454.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
220523s2008 xxk|||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1601-5223 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
https://doi.org/10.1111/j.1601-5223.2008.02046.x |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-5401 |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Zhao Xian-lin |
| 9 (RLIN) |
20761 |
| 245 10 - TITLE STATEMENT |
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| Title |
Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
United Kingdom : |
| Name of publisher, distributor, etc. |
Wiley, |
| Date of publication, distribution, etc. |
2008. |
| 340 ## - PHYSICAL MEDIUM |
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| Material base and configuration |
Computer File|Printed |
| 500 ## - GENERAL NOTE |
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| General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0018-0661 |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 500 ## - GENERAL NOTE |
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| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
The objectives of this study were to clarify the relationship between LMW-GS Glu-D3 gene of Ae. tauschii registered in GenBank and the six Glu-D3 genes including 12 allelic variants of common wheat characterized in our previous studies, and identify novel Glu-D3 genes and haplotypes from Ae. tauschii using gene specific PCR amplification. By searching the NCBI database, 13 LMW-GS genes/pseudogenes of Ae. tauschii were retrieved and classified into five gene families based on their nucleotide similarity with the six Glu-D3 genes of common wheat. Of them, four Ae. tauschii genes, AY585350, AY585354, AY585355 and AY585356 matched GluD3-4, GluD3-5, GluD3-1 and GluD3-2 of common wheat, respectively, and one pseudogene AY585351 matched to GluD3-6, but none of them matched to GluD3-3. In order to identify the Glu-D3 genes from Ae. tauschii corresponding to GluD3-3 and GluD3-6 of common wheat, gene specific primers were developed to amplify 8–18 Ae. tauschii entries. As a result, two novel Glu-D3 genes, designated as GluDt3-3 and GluDt3-6, were identified. GluDt3-3 showed seven allelic variants or haplotypes at the DNA level in eight Ae. tauschii entries, designated as GluDt3-31, GluDt3-32, GluDt3-33, GluDt3-34, GluDt3-35, GluDt3-36 and GluDt3-37, respectively. Two to eight SNPs were found among the seven haplotypes and 1–4 amino acid substitutions among the deduced peptides. Multiple sequence alignments showed that the DNA similarity was 99.6–99.9% among the seven GluDt3-3 haplotypes, and 99.4–99.7% between these haplotypes and those of common wheat GluD3-3 gene. GluDt3-6 presented seven haplotypes in 18 Ae. tauschii entries, designated as GluDt3-61, GluDt3-62, GluDt3-63, GluDt3-64, GluDt3-65, GluDt3-66 and GluDt3-67, respectively. GluDt3-61 from Ae. tauschii entry Ae38 was the only one haplotype with complete coding sequence, and the other six were all pseudogenes. Compared with GluD3-6 gene of common wheat, GluDt3-61 exhibited a 3-bp insertion, a 42-bp deletion and 11 base substitutions, leading to a glutamine insertion in position 52, 14 amino acid deletion in position 84–97 and 10 amino acid mutations in its deduced peptide; GluDt3-62 and GluDt3-63 showed a 6-bp insertion, a 24-bp deletion and 15–21 base substitutions in coding region, of which a nonsense mutation from C to T at position 622 resulted in pseudogenes; GluDt3-64 had five base substitution, including a nonsense mutation at the position 742. GluDt3-65, GluDt3-66 and GluDt3-67 all had a base deletion at position 247, as well as 7–8 base substitutions, which resulted in frameshift mutations in the three haplotypes. The results indicated that Ae. tauschii also contains six Glu-D3 genes and their allelic variants are even richer than those in common wheat. |
| 536 ## - FUNDING INFORMATION NOTE |
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| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
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| Language note |
Text in English |
| 594 ## - STAFFID |
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| StaffID |
INT2411 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
1000 |
| Topical term or geographic name as entry element |
Aegilops |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
2185 |
| Topical term or geographic name as entry element |
Alleles |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1848 |
| Topical term or geographic name as entry element |
Genetic markers |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1937 |
| Topical term or geographic name as entry element |
Nucleotide sequence |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
3635 |
| Topical term or geographic name as entry element |
Mutation |
| Source of heading or term |
AGROVOC |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yan Yang |
| 9 (RLIN) |
8657 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
He Zhonghu |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2411 |
| 9 (RLIN) |
838 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zhensheng Lei |
| 9 (RLIN) |
17779 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wujun Ma |
| 9 (RLIN) |
1979 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Qixin Sun |
| 9 (RLIN) |
22892 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Xianchun Xia |
| 9 (RLIN) |
377 |
| 773 0# - HOST ITEM ENTRY |
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| Title |
Hereditas |
| Note |
635362 |
| Related parts |
v. 145, no. 5, p. 238-250 |
| Place, publisher, and date of publication |
United Kingdom : Wiley, 2008. |
| Record control number |
G444334 |
| International Standard Serial Number |
1601-5223 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
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| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
http://hdl.handle.net/10883/2679 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |