Knowledge Center Catalog

High-lysine corn generated by endosperm-specific suppression of lysine catabolism using RNAi (Record no. 27186)

MARC details
000 -LEADER
fixed length control field 02311nab a22003257a 4500
001 - CONTROL NUMBER
control field G90961
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170719155353.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Houmard, N.M.
245 00 - TITLE STATEMENT
Title High-lysine corn generated by endosperm-specific suppression of lysine catabolism using RNAi
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2007
340 ## - PHYSICAL MEDIUM
Material base and configuration Computer File
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1467-7644
520 ## - SUMMARY, ETC.
Summary, etc. Because of the limited lysine content in corn grain, synthetic lysine supplements are added to corn meal-based rations for animal feed. The development of biotechnology, combined with the understanding of plant lysine metabolism, provides an alternative solution for increasing corn lysine content through genetic engineering. Here, we report that by suppressing lysine catabolism, transgenic maize kernels accumulated a significant amount of lysine. This was achieved by RNA interference (RNAi) through the endosperm-specific expression of an inverted-repeat (IR) sequence targeting the maize bifunctional lysine degradation enzyme, lysine-ketoglutarate reductase/saccharopine dehydrogenase (ZLKR/SDH). Although plant-short interfering RNA (siRNA) were reported to lack tissue specificity due to systemic spreading, we confirmed that the suppression of ZLKR/SDH in developing transgenic kernels was restricted to endosperm tissue. Furthermore, results from our cloning and sequencing of siRNA suggested the absence of transitive RNAi. These results support the practical use of RNAi for plant genetic engineering to specifically target gene suppression in desired tissues without eliciting systemic spreading and the transitive nature of plant RNAi silencing.
546 ## - LANGUAGE NOTE
Language note English
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element high-lysine corn
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Lysine
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element lysine catabolism
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element lysine-ketoglutarate reductase/saccharopine dehydrogenase (LKR/SDH)
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element RNAi
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Bonin, C.P.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Luethy, M.H.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Mainville, J.L.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Malvar, T.M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Shihshieh Huang,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Plant Biotechnology Journal
Related parts v. 5, no. 5, p. 605-614
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/05/2017   07/05/2017 Article Not Lost     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/05/2017

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