MARC details
| 000 -LEADER |
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| fixed length control field |
03199nab a22003977a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G90563 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20230904152542.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
210928s2008 cc |||p|op||| 00| 0 chi d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0578-1752 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
chi |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-5354 |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yingxiu Wan |
| 9 (RLIN) |
5893 |
| 245 10 - TITLE STATEMENT |
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| Title |
Development of multiplex PCR and identification of major quality genes in cultivars from Yellow and Huai River Valley wheat region |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Beijing (China) : |
| Name of publisher, distributor, etc. |
Academy of Agricultural Sciences, |
| Date of publication, distribution, etc. |
2008. |
| 340 ## - PHYSICAL MEDIUM |
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| Material base and configuration |
Computer File|Printed |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 500 ## - GENERAL NOTE |
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| General note |
Abstract in English and Chinese |
| 500 ## - GENERAL NOTE |
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| General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
(Objective) Wheat quality is controlled by many loci and each of them has several alleles. Development of multiplex PCR is very important to enhance the efficiency and reduce the cost in molecular marker assisted breeding for wheat quality traits. (Method) In the present study, the molecular markers for important quality trait genes, i.e., high-molecular-weight glutenin subunit (HMW-GS) genes Ax2*, Bx14, Bx17 and Dx5, and low-molecular-weight glutenin subunit (LMW-GS) gene Glu-A3d, as well as the marker BDFL-BRD for Wx-B1, MAG269 for Wx-D1, ƒÖ-sec for 1BL/1RS translocation and PPO18 for polyphenol oxidase(PPO) activity, were chosen. Three combinations of multiplex PCRs, PCR-I (Ax2*/Bx17/Dx5), PCR-II (Bx14/Glu-A3d/ƒÖ-sec) and PCR- III (BDFL-BRD/MAG269/PPO18) were built up according to genes controlling for pan bread and Chinese noodle quality, and the annealing temperatures of different markers. (Result) A total of 141 wheat cultivars from Yellow and Huai River Valley wheat region were evaluated adopting the multiplex PCRs, and 80 of them were previously assessed using SDS-PAGE for HMW-GS, LMW-GS and secalin, and the corresponding results were identical. Of the 141 wheat cultivars, the frequencies of Ax2*, Bx14 and Bx17 were 4.3%, 7.1%, and 1.4%, while Dx5 and Glu-A3d accounted for 17.7% and 27.7%, respectively. Presences of 1BL/1RS translocation and low polyphenol oxidase (PPO) activity genes were much higher, up to 44.0% and 51.8%, respectively. Wx-B1 null type accounted for 4.3%, and no Wx-D1 null type presented. (Conclusion) The results indicated that the three multiplex PCRs developed can be used to detect the nine genes stably and efficiently. |
| 536 ## - FUNDING INFORMATION NOTE |
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| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
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| Language note |
Text in Chinese |
| 594 ## - STAFFID |
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| StaffID |
INT2411 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Wheat |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1310 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Triticum aestivum |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1296 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Processing quality |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
9916 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
PCR |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
12563 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
16893 |
| Personal name |
Zhang Xiao-Ke |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
377 |
| Personal name |
Xianchun Xia |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5892 |
| Personal name |
Pingzhi Zhang |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
He Zhonghu |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2411 |
| 9 (RLIN) |
838 |
| 773 0# - HOST ITEM ENTRY |
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| Title |
Scientia Agricultura Sinica |
| Note |
635297 |
| Related parts |
v. 41, no. 3, p. 643-653 |
| Place, publisher, and date of publication |
Beijing (China) : Academy of Agricultural Sciences, 2008. |
| Record control number |
G445218 |
| International Standard Serial Number |
0578-1752 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
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| Link text |
Access only for CIMMYT Staff |
| Uniform Resource Identifier |
https://hdl.handle.net/20.500.12665/1527 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |