Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) (Record no. 27048)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03226nab a22004697a 4500 |
| 001 - CONTROL NUMBER | |
| control field | G90497 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220516154528.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220516s2008 xxu|||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 0733-5210 |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1016/j.jcs.2007.04.013 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | CIS-5330 |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Qiang Zhang |
| 9 (RLIN) | 6993 |
| 245 10 - TITLE STATEMENT | |
| Title | Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | USA : |
| Name of publisher, distributor, etc. | Elsevier, |
| Date of publication, distribution, etc. | 2008. |
| 340 ## - PHYSICAL MEDIUM | |
| Material base and configuration | Printed |
| 500 ## - GENERAL NOTE | |
| General note | Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210 |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The sample preparation method of high molecular weight glutenin subunits (HMW-GS) for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, without a separation step, by high-performance liquid chromatography (HPLC) was established in this study. Three major factors influencing mass spectra—the ratio of components of the solvent, the resolving time, and the sample volume—were optimized using HMW-GS mixtures extracted from Chinese cultivar Jing 411. The results showed that the optimized method for sample preparation was to resolve HMW-GS from 20 mg in an hour with 50 μl solvent of 0.4% TFA, 30.0% ACN and 69.6% H2O. The stable mass spectra and accurate molecular weights of 16 major HMW glutenin subunits from common wheat and related species were obtained using the optimized MALDI-TOF-MS method. Seven subunits, where each was from 2–5 cultivars, showed very similar molecular weights. The determined molecular weights of 11 subunits were close to those calculated from their coding sequences. In addition, no positive reaction between HMW-GS and GelCode® Glycoprotein Staining Reagent was observed. These results suggested that HMW-GS lack extensive post-translational modifications (PTMs), but low levels of glycosylation or phosphorylation present in some subunits cannot be ruled out. Because of its ability to obtain a rapid, complete and precise profile of HMW glutenin subunits without purifying procedures, MALDI-TOF-MS is expected to be a powerful technique for structural and functional studies of HMW glutenin subunits as well as other cereal proteins. |
| 536 ## - FUNDING INFORMATION NOTE | |
| Text of note | Global Wheat Program |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 591 ## - CATALOGING NOTES | |
| Affiliation | Elsevier |
| 594 ## - STAFFID | |
| StaffID | INT2411 |
| 595 ## - COLLECTION | |
| Collection | CIMMYT Staff Publications Collection |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular weight |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1184 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Mass spectrometry |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 11789 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Glutenins |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1137 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Wheat |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1310 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yanmin Dong |
| 9 (RLIN) | 27463 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xueli An |
| 9 (RLIN) | 27464 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aili Wang |
| 9 (RLIN) | 20799 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yanzhen Zhang |
| 9 (RLIN) | 24260 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xiaohui Li |
| 9 (RLIN) | 18575 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liyan Gao |
| 9 (RLIN) | 20798 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xianchun Xia |
| 9 (RLIN) | 377 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | He Zhonghu |
| Miscellaneous information | Global Wheat Program |
| Field link and sequence number | INT2411 |
| 9 (RLIN) | 838 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yueming Yan |
| 9 (RLIN) | 15752 |
| 773 0# - HOST ITEM ENTRY | |
| Title | Journal of Cereal Science |
| Note | 635225 |
| Related parts | v. 47, no. 2, p. 252-261 |
| Place, publisher, and date of publication | USA : Elsevier, 2008. |
| Record control number | G444514 |
| International Standard Serial Number | 0733-5210 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Full call number | Barcode | Copy number | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 02/10/2015 | CIS-5330 | 635225 | 1 | 02/10/2015 | Article | Not Lost | Dewey Decimal Classification | CIMMYT Staff Publications Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 02/10/2015 |