MARC details
| 000 -LEADER |
|---|
| fixed length control field |
04957nab a22004577a 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
G90246 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20220518174749.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
220518s2007 cc |||p|op||| 00| 0 chi d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1875-2780 (Online) |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
0496-3490 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
chi |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-5163 |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hao Li |
| 9 (RLIN) |
18205 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Isolation of differentially expressed genes from wheat cultivars Jinan 17 and Yumai 34 with good bread quality under heat stress during grain filling stage |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Beijing, (China) : |
| Name of publisher, distributor, etc. |
Institute of Crop Sciences, |
| Date of publication, distribution, etc. |
2007. |
| 340 ## - PHYSICAL MEDIUM |
|---|
| Material base and configuration |
Computer File|Printed |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Abstract in Chinese and English. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer-review: No - Open Access: Yes|http://211.155.251.148:8080/zwxb/EN/column/column81.shtml |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Studies of gene expression patterns under heat stress during grain filling stage will provide important information for breeding wheat cultivars with high quality. In the present study, two wheat cultivars, Jinan 17 and Yumai 34 with different quality stability under various environments, were used to in the influence of high temperature on gene expression. The wheat plants were exposed to high temperature (38℃/25℃ day/night) for three days in the middle (from 15 to 18 days post-anthesis) and late stage (from 30 to 33 days post-anthesis) of grain filling in a climate chamber. Spikelets in middle of heads were harvested, and RNA of kernels was extracted with a combined technique of cold phenolic and Trizol single-step methods. cDNA was obtained by the reverse transcription of total RNA, and differential bands were detected subsequently in cDNA-AFLP analysis. In total, 410 and 316 differential bands were detected from Jinan 17 and Yumai 34, respectively. The differential fragments were cloned, sequenced and blasted in NCBI, and 85 and 99 positive fragments of differentially expressed genes under heat stress were obtained from Jinan 17 and Yumai 34, respectively. After the positive fragments were validated by reverse Northern blotting, 25 positive fragments isolated from Jinan 17 showed intense signal, and 22 of them were induced under heat stress, which were notablely homologous to stress response genes and heat shock protein of wheat. Meanwhile, 31 positive fragments showed intense signal were observed from Yumai 34, and 25 of them were suppressed, which were notablely homologous to stress response genes, ethylene forming enzyme, pyrroline-5-carboxylate synthetase. The rusults indicated that gene expression was more induced under heat stress in Jinan17, whereas suppressed more in Yumai 34, which might lead to differences in heat tolerance and quality stability. Five fragments from Jinan 17, and two fragments from Yumai 34 did not have any homology sequences in the BLAST analysis, while other fragments have homology protein or nucleic acid sequences in wheat or other crops. Two fragments induced in response to heat stress were notablely homologous to the storage protein genes, which might induce the expression of transcripts related to storage protein under heat stress. Fifteen differential fragments were detected from medium stage of grain filling in Jinan 17, whereas those from late stage were 10, while 29 and 2 differential fragments in Yumai 34 were observed in medium and late stages, respectively. This indicated that gene expression was more significantly affected by heat stress in medium stage than in late stage of grain filling, especially in Yumai 34. The difference of gene expression patterns between two wheat cultivars were observed, stress response genes were induced in Jinan 17, ethylene forming enzyme and pyrroline-5-carboxylate synthetase as well as stress response genes were suppressed in Yumai 34, which may result in the different responses in heat tolerance and quality stability. The identification and characterization of heat stress responsive genes in wheat may provide a molecular biological understanding of gene expression patterns and regulation involved in the heat stress in wheat. |
| 536 ## - FUNDING INFORMATION NOTE |
|---|
| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in Chinese |
| 594 ## - STAFFID |
|---|
| StaffID |
INT2411 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Wheat |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1310 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Seed filling |
| 9 (RLIN) |
1911 |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Heat stress |
| 9 (RLIN) |
1971 |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Amplified fragment length polymorphism |
| 9 (RLIN) |
13624 |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
8835 |
| Topical term or geographic name as entry element |
Gene Expression |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Quality |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1231 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Stability |
| 9 (RLIN) |
6345 |
| Source of heading or term |
AGROVOC |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zhang Ping-ping |
| 9 (RLIN) |
23183 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zha Xiang-Dong |
| 9 (RLIN) |
27515 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xianchun Xia |
| 9 (RLIN) |
377 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
He Zhonghu |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2411 |
| 9 (RLIN) |
838 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Acta Agronomica Sinica |
| Note |
635011 |
| Related parts |
v. 33, no. 10, p. 1644-1653 |
| Place, publisher, and date of publication |
Beijing, (China) : Institute of Crop Sciences, 2007. |
| Record control number |
G446116 |
| International Standard Serial Number |
0496-3490 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
http://hdl.handle.net/10883/2612 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |