MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03479nab a22004577a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G90240 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20230912212610.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
210702b2007 xxc|||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1480-3321 (Online) |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
0831-2796 |
| 024 8# - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
https://doi.org/10.1139/G07-089 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-5157 |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xiaohui Li |
| 9 (RLIN) |
18575 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Molecular cloning, heterologous expression, and phylogenetic analysis of a novel y-type HMW glutenin subunit gene from the G genome of Triticum timopheevi |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Ontario (Canada) : |
| Name of publisher, distributor, etc. |
Canadian Science Publishing, |
| Date of publication, distribution, etc. |
2007. |
| 340 ## - PHYSICAL MEDIUM |
|---|
| Material base and configuration |
Printed|Computer File |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0831-2796 |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
A novel y-type high molecular weight (HMW) glutenin subunit gene from the G genome of Triticum timopheevi (2n = 4x = 28, AAGG) was isolated and characterized. Genomic DNA from accession CWI17006 was amplified and a 2200 bp fragment was obtained. Sequence analysis revealed a complete open reading frame including N- and C-terminal ends and a central repetitive domain encoding 565 amino acid residues. The molecular weight of the deduced subunit was 77 031, close to that of the x-type glutenin subunits. Its mature protein structure, however, demonstrated that it was a typical y-type HMW subunit. To our knowledge, this is the largest y-type subunit gene among Triticum genomes. The molecular structure and phylogenetic analysis assigned it to the G genome and it is the first characterized y-type HMW glutenin subunit gene from T. timopheevi. Comparative analysis and secondary structure prediction showed that the subunit possessed some unique characters, especially 2 large insertions of 45 (6 hexapeptides and a nonapeptide) and 12 (2 hexapeptides) amino acid residues that mainly contributed to its higher molecular weight and allowed more coils to be formed in its tertiary structure. Additionally, more α-helixes in the repeat domain of the subunit were found when compared with 3 other y-type subunits. We speculate that these structural characteristics improve the formation of gluten polymer. The novel subunit, expressed as a fusion protein in E. coli, moved more slowly in SDS–PAGE than the subunit Bx7, so it was designated Gy7*. As indicated in previous studies, increased size and more numerous coils and α-helixes of the repetitive domain might enhance the functional properties of HMW glutenins. Consequently, the novel Gy7* gene could have greater potential for improving wheat quality. |
| 536 ## - FUNDING INFORMATION NOTE |
|---|
| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 594 ## - STAFFID |
|---|
| StaffID |
INT2411 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Wheat |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1310 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Glutenins |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1137 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Triticum timopheevii |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
31621 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genomes |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1131 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zhang Yan-zhen |
| 9 (RLIN) |
20781 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Liyan Gao |
| 9 (RLIN) |
20798 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Aili Wang |
| 9 (RLIN) |
20799 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kangmin Ji |
| 9 (RLIN) |
20800 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
He Zhonghu |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2411 |
| 9 (RLIN) |
838 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
1980 |
| Personal name |
Appels, R. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
1979 |
| Personal name |
Wujun Ma |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
15752 |
| Personal name |
Yueming Yan |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Genome |
| Note |
635005 |
| Related parts |
v. 50, no. 12, p. 1130-1140 |
| Place, publisher, and date of publication |
Ontario (Canada) : Canadian Science Publishing, 2007. |
| Record control number |
G444552 |
| International Standard Serial Number |
0831-2796 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Access only for CIMMYT Staff |
| Uniform Resource Identifier |
https://hdl.handle.net/20.500.12665/649 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |