Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance (Record no. 26915)

000 -LEADER
fixed length control field 03279nab a22004577a 4500
001 - CONTROL NUMBER
control field a90237
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20171107160103.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-5154
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Yang, Y.
245 00 - TITLE STATEMENT
Title Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2007
340 ## - PHYSICAL MEDIUM
Material base and configuration Computer File|Printed
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0022-0957
520 ## - SUMMARY, ETC.
Summary, etc. Pre-harvest sprouting (PHS) of wheat reduces the quality and economic value of grain, and increasing PHS tolerance is one of the most important traits in wheat breeding. Two new Vp-1B alleles related to PHS tolerance were identified on the 3BL chromosome of bread wheat and were designated Vp-1Bb and Vp-1Bc. Sequence analysis showed that Vp-1Bb has a 193 bp insertion and Vp-1Bc has a 83 bp deletion located in the third intron region of the Vp-1B gene, and that they shared 95.43% and 97.89% similarity, respectively, with the sequence of AJ400713 (Vp-1Ba) at the nucleotide level. Their sequences were deposited in the GenBank under the accession numbers DQ517493 and DQ517494. Semi-quantitative RT-PCR analysis showed that alternatively spliced transcripts of the Vp-1A, Vp-1B, and Vp-1D homologues were present and there were no differences in the splicing patterns or abundances of Vp-1A and Vp-1D from embryos 35 d after pollination between PHS-tolerant and -susceptible cultivars. Although Vp-1Ba, Vp-1Bb, and Vp-1Bc could each produce a set of transcripts, only one was correctly spliced and had the capacity to encode the full-length VP1 protein and was more highly expressed with Vp-1Bb and Vp-1Bc than with Vp-1Ba. Comparison of the expression patterns of Vp-1Ba, Vp-1Bb, and Vp-1Bc on different days after pollination also revealed that the expression of these genes was developmentally regulated. Furthermore, genotypes with different levels of tolerance to PHS respond differently to ABA exposure and differences in transcript levels of Vp-1Ba, Vp-1Bb, and Vp-1Bc were observed after ABA treatment. The results indicated that insertion or deletion in the third intron region might affect the expression of the Vp-1B gene and its sensitivity to ABA, and thus resistance to PHS.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation Oxford
594 ## - STAFFID
StaffID INT2411
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element ABA responsiveness
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element allele
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Expression
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element pre-harvest sprouting
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element RT-PCR
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Triticum aestivum L
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Vp-1
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Chen, X.M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Jones, H.D.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Ma, Y.Z.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Shewry, P.R.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Wilkinson, M.,
Relator term coaut.
9 (RLIN) 2577
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Xia, L.Q.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Xu, Z.S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Yu, Z.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 838
Personal name He Zhonghu
Miscellaneous information Global Wheat Program
Field link and sequence number INT2411
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Journal of Experimental Botany
Note 635002
Related parts v. 58, no. 11, p. 2863-2871
856 4# - ELECTRONIC LOCATION AND ACCESS
Link text Access only for CIMMYT Staff
Uniform Resource Identifier http://46.20.115.203/download/cis/90237.pdf
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme
Holdings
Withdrawn status Lost status Damaged status Not for loan Collection code Permanent Location Current Location Date acquired Full call number Barcode Date last seen Copy number Price effective from Koha item type
  Not Lost     CIMMYT Staff Publications Collection CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 2015-02-10 CIS-5154 635002 2015-02-10 1 2015-02-10 Article
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Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
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