Knowledge Center Catalog

Electroporation of maize embryogenic calli with the trehalose-6-phosphate synthase gene from Arabidopsis thaliana (Record no. 26887)

MARC details
000 -LEADER
fixed length control field 02548nab a22003257a 4500
001 - CONTROL NUMBER
control field G90168
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20211006085005.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) REP-12964
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Almeida, A.M.
245 00 - TITLE STATEMENT
Title Electroporation of maize embryogenic calli with the trehalose-6-phosphate synthase gene from Arabidopsis thaliana
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2007
340 ## - PHYSICAL MEDIUM
Material base and configuration Computer File|Printed
520 ## - SUMMARY, ETC.
Summary, etc. Trehalose is a non-reducing disaccharide of glucose that occurs in a large number of organisms, playing an important role in desiccation and heat stress protection. Trehalose accumulation has proven to be an effective way of increasing drought tolerance in both model plants such as tobacco and important crops such as potato or rice. In this work we aim to genetically engineer maize with the Arabidopsis thaliana trehalose phosphate synthase gene (AtTPS1), involved in trehalose biosynthesis via electroporation. A cassette harboring the AtTPS1 gene under the control of the CaMV35S promoter and the Bialaphos resistance gene Bar as a selective agent was inserted in the plasmid vector pGreen0229 and used to transform maize inbred line Pa91 via electroporation. Fifteen putative transgenic plants (T0 generation) were obtained. Transgene integration in T0 plants was analyzed by Southern-blot analysis. T0 plants had normal phenotypes, although smaller than wild type plants. Contrary to wild type plants, when sexual organs emerged, tassels appeared at least 15 days earlier than ears in the same plant, rendering impossible the self-pollination of the T0 plant. These plants were then crossed with wild type plants and in some cases T1 seeds were obtained. T1 seeds presented deformities, especially the lack of endosperm, but it was still possible to germinate some of these seeds. The so obtained plants were tested by Northern blot but no AtTPS1 gene expression was detected, a fact possibly due to the incomplete insertion of the AtTPS1 gene or an extremely low gene expression level.
546 ## - LANGUAGE NOTE
Language note English
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Electroporation
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Maize
Miscellaneous information AGROVOC
Source of heading or term
9 (RLIN) 1173
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Trehalose
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Araujo, S.S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Cardoso, L.A.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Fevereiro, P.S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Santos, D.M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Santos, M.A.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Torne, J.M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Villalobos, E.,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Acta Physiologiae Plantarum
Note 634941
Related parts v. 29, no. 3, p. 273-281
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Full call number Barcode Copy number Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
02/10/2015   REP-12964 634941 1 02/10/2015 Article Not Lost     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 02/10/2015

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