MARC details
| 000 -LEADER |
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| fixed length control field |
02584nab a22003737a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G89947 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20230914204317.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
210920s2007 cc |||p|op||| 00| 0 chi d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0578-1752 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
chi |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-5020 |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zhang Ping-ping |
| 9 (RLIN) |
23183 |
| 245 10 - TITLE STATEMENT |
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| Title |
Protocol establishment of reversed-phase high-performance liquid chromatography (RP-HPLC) for analyzing wheat gluten protein |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Beijing (China) : |
| Name of publisher, distributor, etc. |
Academy of Agricultural Sciences, |
| Date of publication, distribution, etc. |
2007. |
| 340 ## - PHYSICAL MEDIUM |
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| Material base and configuration |
Computer File|Printed |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 500 ## - GENERAL NOTE |
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| General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
(Objective) This study will establish a protocol of reversed-phase high-performance liquid chromatography (RP-HPLC) used in separating and quantifying wheat gluten protein in China, which has been widely used in wheat quality study since the 1980s. (Method) Two cultivars, Zhongyou 9507 (strong gluten) and Jing 411 (weak gluten), were chosen to study the effect of temperature, elution gradient, sample weight, extraction duration, and injection volume on gluten protein resolution. The ability to repeat experimental results was validated. (Result) The results indicated that the gliadin and glutenin subunits extraction rate reached 90% when 45mg of flour was extracted with 1 ml 50% aqueous 1-propanol (v/v) and glutenin extraction buffer (50% 1-propanal (v/v) +2 mol L-1 Urea+0.2 mol L-1 Tris-HCl (pH 6.6)+1%DTT), respectively. The optimal sample injection volume of gliadin and glutenin was 10-15 and 15-20, respectively. The modified elution gradient effectively separated gliadin and glutenin subunits. (Conclusion) This protocol can qualify and quantify the components of gliadin and glutenin accurately combining SDS-PAGE, by which we can predicate the wheat end-use quality. |
| 536 ## - FUNDING INFORMATION NOTE |
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| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
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| Language note |
Text in Chinese |
| 594 ## - STAFFID |
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| StaffID |
INT2411 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Wheat |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1310 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Gluten |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1974 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Proteins |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1224 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
HPLC |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
6176 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yong Zhang |
| 9 (RLIN) |
1857 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xianchun Xia |
| 9 (RLIN) |
377 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
He Zhonghu |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2411 |
| 9 (RLIN) |
838 |
| 773 0# - HOST ITEM ENTRY |
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| Title |
Scientia Agricultura Sinica |
| Note |
634771 |
| Related parts |
v. 40, no. 5, p. 1002-1009 |
| Place, publisher, and date of publication |
Beijing (China) : Academy of Agricultural Sciences, 2007. |
| Record control number |
G445218 |
| International Standard Serial Number |
0578-1752 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
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| Link text |
Access only for CIMMYT Staff |
| Uniform Resource Identifier |
https://hdl.handle.net/20.500.12665/1428 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |