MARC details
| 000 -LEADER |
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| fixed length control field |
02078nab a22003737a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G89702 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20220511204025.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
220511s2006 nr |||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1684-5315 |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Source of number or code |
https://doi.org/10.5897/AJB06.431 |
| Qualifying information |
DOI is wrong in original article |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-4930 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Danson, J. |
| 9 (RLIN) |
7404 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Detection of Bacillus thuringiensis genes in transgenic maize by the PCR method and FTA paper technology |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Nigeria : |
| Name of publisher, distributor, etc. |
Academic Journals, |
| Date of publication, distribution, etc. |
2006. |
| 340 ## - PHYSICAL MEDIUM |
|---|
| Material base and configuration |
Computer File|Printed |
| 500 ## - GENERAL NOTE |
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| General note |
Peer-review: No - Open Access: Yes|http://www.ajol.info/index.php/ajb |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
We optimized the PCR method to detect genetically engineered Bacillus thuringiensis (Bt) maize in open quarantine fields in Kenya. Many factors affect the extraction of the DNA from plants, such as the amount of tissue available, the condition of the plant material, the numbers of steps involved in the extraction procedure, and the required purity needed. We tested the application of the FTA paper technology for field sampling whereby leaf materials are not removed and transported from the site. We also applied the PCR method, a technique that is widely used for detection of genetically modified organism (GMOs). We used primers specific to the Bt genes present in the transgenic maize to screen for the Bt genes Cry 1Ab and Cry 1Ba. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 591 ## - CATALOGING NOTES |
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| Affiliation |
Academic Journals |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genetically modified organisms |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
10536 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Maize |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Bacillus thuringiensis |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
6672 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
PCR |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
12563 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kimani, M., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mbogori, M., |
| Relator term |
coaut. |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
African Journal of Biotechnology |
| Note |
634572 |
| Related parts |
v. 5, no. 22, p. 2345-2349 |
| Place, publisher, and date of publication |
Nigeria : Academic Journals, 2006. |
| Record control number |
G76647 |
| International Standard Serial Number |
1684-5315 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
http://hdl.handle.net/10883/3034 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |