MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03104nab a22004097a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G89700 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20220519223240.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
220519s2006 nr |||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1684-5315 |
| 024 8# - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
https://doi.org/10.5897/AJB06.499 |
| Qualifying information |
DOI is wrong in original article |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-4928 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Danson, J. |
| 9 (RLIN) |
7404 |
| 245 10 - TITLE STATEMENT |
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| Title |
Marker assisted introgression of opaque2 gene into herbicide resistant elite maize inbred lines |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Nigeria : |
| Name of publisher, distributor, etc. |
Academic Journals, |
| Date of publication, distribution, etc. |
2006. |
| 340 ## - PHYSICAL MEDIUM |
|---|
| Material base and configuration |
Printed|Computer File |
| 500 ## - GENERAL NOTE |
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| General note |
Peer-review: No - Open Access: Yes|http://www.ajol.info/index.php/ajb |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Marker assisted selection in combination with conventional breeding can greatly accelerate the introgression of modified opaque2 genotype into herbicide resistant maize. By combining these two approaches, time and costs are greatly minimized. The application of opaque2 allele specific SSR markers was done on materials already undergoing selection in a breeding program for converting herbicide resistant maize lines into quality protein maize (QPM) which is the equivalent of modified opaque2 phenotype. The breeder had selected QPM lines using the light table in the previous cycle and we used leaf samples to extract DNA for analysis of the presence of the opaque2 gene using SSR markers. Two co-dominant SSR markers phi057 and umc1066 and a dominant marker phi112 were used. Umc1216, a modifier marker was also tested in combination with the opaque2 markers with the objective of using the marker to select for modifiers for the opaque2 phenotype. The modified FTA paper technology protocol was applied in field sampling. The results showed 97% of the lines were opaque2 while 3% were non-opaque2. Both methods of conventional breeding using light table and marker assisted selection (MAS) were comparable. However, the application of SSR markers and the FTA technology offers the breeder a fast, time saving, reliable and less labour intensive method of screening QPM maize during the early growing stages instead of having to wait to screen the kernels on the light table after harvesting. Moreover, the routine biochemical analysis for high lysine and tryptophan levels need not be carried out at each backcross since the presence of the opaque2 gene is confirmed with markers. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
Text in English |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Academic Journals |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genetic markers |
| 9 (RLIN) |
1848 |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Maize |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Protein quality |
| 9 (RLIN) |
1223 |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Marker-assisted selection |
| 9 (RLIN) |
10737 |
| Source of heading or term |
AGROVOC |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mbogori, M. |
| 9 (RLIN) |
27566 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kimani, M. |
| 9 (RLIN) |
27567 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lagat, M. |
| 9 (RLIN) |
27565 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kuria, A. |
| 9 (RLIN) |
27568 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
69 |
| Personal name |
Diallo, A. |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
African Journal of Biotechnology |
| Note |
634570 |
| Related parts |
v. 5, no. 24, p. 2417-2422 |
| Place, publisher, and date of publication |
Nigeria : Academic Journals, 2006. |
| Record control number |
G76647 |
| International Standard Serial Number |
1684-5315 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
http://hdl.handle.net/10883/3032 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |