Knowledge Center Catalog

Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat (Record no. 26292)

MARC details
000 -LEADER
fixed length control field 03263nab a22004697a 4500
001 - CONTROL NUMBER
control field G89250
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20230918195552.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210701s2006 xxu|||p|op||| 00| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0733-5210
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1016/j.jcs.2005.08.003
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-4766
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Zhensheng Lei
9 (RLIN) 17779
245 10 - TITLE STATEMENT
Title Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. USA :
Name of publisher, distributor, etc. Elsevier,
Date of publication, distribution, etc. 2006.
340 ## - PHYSICAL MEDIUM
Material base and configuration Printed|Computer File
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210
520 ## - SUMMARY, ETC.
Summary, etc. A number of primers were designed which target DNA sequence variation of the coding and /or promoter regions of wheat HMW glutenin y-type genes located at the Glu-B1 locus. This allowed the development of a set of PCR-based markers for specific HMW glutenin genes encoding By-subunits for which no markers were previously available. Markers were validated using test cultivars containing specific Glu-B1 alleles confirmed by SDS-PAGE and RP-HPLC analysis. Among the specific markers developed, primer pair ZSBy8F5/R5 was specific for the By8 gene, which exists in Glu-B1b (Bx7+By8) and Glu-B1u (Bx7*+By8) alleles. This marker allows discrimination of alleles containing By8 and By8* that are usually difficult to distinguish using SDS-PAGE. Since the over-expressed Glu-B1 allele (Glu-Bl al.) contains the By8* subunit, it is possible to use this marker in breeding programs for selecting for the over-expression of subunit Bx7 in crosses that segregate between normal Bx7 and over-expressed Bx7 subunits. This marker also represents an alternative for distinguishing two common Glu-B1 alleles: Glu-B1i (Bx17+By18) and Glu-B1b (Bx7+By8). Two primer pairs ZSBy9aF1/R3 and ZSBy9F7/R6 both gave characteristic banding patterns for Glu-B1c (Bx7+By9) and can therefore be used to discriminate By9 - containing alleles from non - By9 alleles. Primer pair ZSBy9F2/R2 produced amplicons with a diagnostic banding pattern for allele Glu-B1f (Bx13+By16) and also permitted the discrimination of Glu-B1h (Bx14+By15) and Glu-B1e (Bx20) that have opposing genetic effects on wheat quality and are difficult to discriminate by SDS-PAGE.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note Text in English
591 ## - CATALOGING NOTES
Affiliation Elsevier|0606
594 ## - STAFFID
StaffID INT2411
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 1310
Topical term or geographic name as entry element Wheat
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 2020
Topical term or geographic name as entry element Hexaploidy
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 1231
Topical term or geographic name as entry element Quality
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 1137
Topical term or geographic name as entry element Glutenins
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 12563
Topical term or geographic name as entry element PCR
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 1848
Topical term or geographic name as entry element Genetic markers
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Gale, K.
9 (RLIN) 13592
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name He Zhonghu
Miscellaneous information Global Wheat Program
Field link and sequence number INT2411
9 (RLIN) 838
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 20756
Personal name Gianibelli, C.
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 20757
Personal name Larroque, O.
700 0# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 377
Personal name Xianchun Xia
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 20758
Personal name Butow, B.J.
700 0# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 1979
Personal name Wujun Ma
773 0# - HOST ITEM ENTRY
Title Journal of Cereal Science
Note 634186
Related parts v. 43, no. 1, p. 94-101
Place, publisher, and date of publication USA : Elsevier, 2006.
Record control number G444514
International Standard Serial Number 0733-5210
856 4# - ELECTRONIC LOCATION AND ACCESS
Link text Access only for CIMMYT Staff
Uniform Resource Identifier https://hdl.handle.net/20.500.12665/569
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme Dewey Decimal Classification
Suppress in OPAC No
Holdings
Date last seen Total Checkouts Full call number Barcode Copy number Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
02/10/2015   CIS-4766 634186 1 02/10/2015 Article Not Lost Dewey Decimal Classification     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 02/10/2015

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