MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03161nab a22004217a 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
G83021 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20171220113520.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
121211b |||p||p||||||| |z||| | |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1432-2242 (Revista en electrónico) |
| 022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
0040-5752 |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1007/s00122-004-1871-x |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
En |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
REP-12519 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Song, Q.J. |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Development and mapping of microsatellite (SSR) markers in wheat |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Date of publication, distribution, etc. |
2005 |
| 340 ## - PHYSICAL MEDIUM |
|---|
| Material base and configuration |
Printed|Computer File |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer-review: Yes - Open Access: Yes |http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752 |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Microsatellite DNA markers are consistently found to be more informative than other classes of markers in hexaploid wheat. The objectives of this research were to develop new primers flanking wheat microsatellites and to position the associated loci on the wheat genome map by genetic linkage mapping in the ITMI W7984 × Opata85 recombinant inbred line (RIL) population and/or by physical mapping with cytogenetic stocks. We observed that the efficiency of marker development could be increased in wheat by creating libraries from sheared rather than enzyme-digested DNA fragments for microsatellite screening, by focusing on microsatellites with the [ATT/TAA] n motif, and by adding an untemplated G-C clamp to the 5′-end of primers. A total of 540 microsatellite-flanking primer pairs were developed, tested, and annotated from random genomic libraries. Primer pairs and associated loci were assigned identifiers prefixed with BARC (the acronym for the USDA-ARS Beltsville Agricultural Research Center) or Xbarc, respectively. A subset of 315 primer sets was used to map 347 loci. One hundred and twenty-five loci were localized by physical mapping alone. Of the 222 loci mapped with the ITMI population, 126 were also physically mapped. Considering all mapped loci, 126, 125, and 96 mapped to the A, B, and D genomes, respectively. Twenty-three of the new loci were positioned in gaps larger than 10 cM in the map based on pre-existing markers, and 14 mapped to the ends of chromosomes. The length of the linkage map was extended by 80.7 cM. Map positions were consistent for 111 of the 126 loci positioned by both genetic and physical mapping. The majority of the 15 discrepancies between genetic and physical mapping involved chromosome group 5. |
| 536 ## - FUNDING INFORMATION NOTE |
|---|
| Text of note |
Genetic Resources Program|Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
English |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Springer|0406|No CIMMYT affiliation |
| 594 ## - STAFFID |
|---|
| StaffID |
INT2733|INT3098 |
| 595 ## - COLLECTION |
|---|
| Collection |
Reprints Collection |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Costa, J.M., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Cregan, P.B., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Fickus, E.W., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Gill, B.S., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lewis, J., |
| Relator term |
coaut. |
| 9 (RLIN) |
2354 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Shi, J.R, |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
892 |
| Personal name |
Sukhwinder-Singh |
| Miscellaneous information |
Genetic Resources Program |
| Field link and sequence number |
INT3098 |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
856 |
| Personal name |
Ward, R.W. |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2733 |
| Relator term |
coaut. |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Theoretical and Applied Genetics |
| Note |
633946 |
| Related parts |
v. 110, no. 3, p. 550-560 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |