MARC details
| 000 -LEADER |
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| fixed length control field |
02489nab a22003737a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G82972 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20211006082400.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
210630s2007 cc |||p|op||| 00| 0 chi d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0578-1752 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
chi |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-4624 |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Genying Li |
| 9 (RLIN) |
5949 |
| 245 10 - TITLE STATEMENT |
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| Title |
Construction of expression vector harboring Pina & Pinb fused gene and transformation into durum wheat |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Beijing (China) : |
| Name of publisher, distributor, etc. |
Academy of Agricultural Sciences, |
| Date of publication, distribution, etc. |
2007. |
| 340 ## - PHYSICAL MEDIUM |
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| Material base and configuration |
Computer File|Printed |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 500 ## - GENERAL NOTE |
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| General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
[Objective] Construction of expression vector harboring the fragment of fused Pina and Pinb, and verification of Pina and Pinb function. [Method] In the present study, a highly efficient expression vector (pFUPaPb) that harbors the fused fragment of Pina Pinb flanked with regulation sequences of and poly (A) was constructed, employing Ubiqutin as a promoter and bar gene as a selective marker. Common wheat variety Jing 411 is the donor of Pina and Pinb genes. pFUPaPb was transferred into durum wheat by biolistic method. [Result] After screening with bialophos and PCR identification with gene specific primers and dot blotting confirmation, 35 transplants were obtained. The expression of fused genes was detected in 2 of 16 transplants examined. The SKCS values of these two lines were decreased 10 and 12, respectively. Among the three medium used for callus inducing SD2 medium was the comparatively efficient medium for callus induction of durum immature embryo. MS+8 mgL-1 zeatin is good for callus regeneration. [Conclusion] Co-expression of Pina and Pinb decreased the endosperm texture of durum wheat. |
| 536 ## - FUNDING INFORMATION NOTE |
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| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
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| Language note |
Text in Chinese |
| 594 ## - STAFFID |
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| StaffID |
INT2411 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Durum wheat |
| 9 (RLIN) |
1086 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Immature embryo culture |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Pina Pinb fused expression vector |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Transformation |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lanqin Xia |
| 9 (RLIN) |
20737 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xianchun Xia |
| 9 (RLIN) |
377 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
He Zhonghu |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2411 |
| 9 (RLIN) |
838 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Scientia Agricultura Sinica |
| Note |
633897 |
| Related parts |
v. 40, no. 7, p. 1315-1323 |
| Place, publisher, and date of publication |
Beijing (China) : Academy of Agricultural Sciences, 2007. |
| Record control number |
G445218 |
| International Standard Serial Number |
0578-1752 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
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| Link text |
Access only for CIMMYT Staff |
| Uniform Resource Identifier |
https://hdl.handle.net/20.500.12665/546 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |