MARC details
| 000 -LEADER |
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| fixed length control field |
02797nab a22004697a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G65469 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20170719110412.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
121211b |||p||p||||||| |z||| | |
| 022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0003-9861 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
95-099007 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Pascal, S. |
| 245 00 - TITLE STATEMENT |
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| Title |
Plant sterol biosynthesis: |
| Remainder of title |
identification of a NADPH dependent sterone reductase involved in sterol-4 demethylation |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Date of publication, distribution, etc. |
1994 |
| 340 ## - PHYSICAL MEDIUM |
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| Material base and configuration |
Printed |
| 500 ## - GENERAL NOTE |
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| General note |
references US (DNAL 381 Ar2) |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Microsomes obtained from maize embryos were shown to catalyze the reduction of various sterones to produce stereoselectively the corresponding 3,6-hydroxy derivatives. Enzymatic assay conditions have been developed to characterize this reduction step and the kinetics of the microsomal system has been established. Sterone reduction shows exclusive dependence on NADPH and is inactive with NADH. It is not sensitive to the azole inhibitors pyrifenox, ketoconazole, and itraconazole nor to phenobarbital nor pyrazole. Based on these coenzyme requirements and inhibitor susceptibility, and according to the common pattern of their classification, the maize microsomal sterone-reducing enzyme belongs to the family of ketone reductases. From a series of incubations with natural or synthetic sterones, the substrate specificity of the reduction at C-3 was determined. Our data indicate particularly that 4 alpha-methyl-9,6,19-cyclo-C30-sterones and 4-desmethyl-delta 7-C27- or C30-sterones are preferentially reduced, while 4,4-dimethyl-C30- or C31-sterones react poorly. The results support the conclusion that the reductase activity identified is a constitutive component of the microsomal sterol 4-demethylation complex recently identified in photosynthetic organisms (S. Pascal et al., 1993, J. Biol. Chem. 268, 11639). They are consistent with the conclusion that 4 alpha-methylsterones are demethylation products of 4,4-gem-dimethylsterols rather than early intermediates in the 4 alpha-monomethylsterols-4-demethylation process |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 595 ## - COLLECTION |
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| Collection |
AGRIS Collection |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Biochemical reactions |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Carbohydrates |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Cell structure |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Chemical reactions |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Cofactors |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Cytoplasm |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Developmental stages |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Enzymes |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Glycosides |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Gramineae |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Nucleic compounds |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Plant developmental stages |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Plant physiology and biochemistry |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Protoplasm |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Steroids |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Sterols |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Zea |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rahier, A., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Taton, M., |
| Relator term |
coaut. |
| 773 0# - HOST ITEM ENTRY |
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| Title |
Archives of biochemistry and biophysics (USA). (Jul 1994). v. 312(1) p. 260-271 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Article |