MARC details
| 000 -LEADER |
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| fixed length control field |
02829nab a22005537a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G65441 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20211006080752.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
121211b |||p||p||||||| |z||| | |
| 022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0099-2240 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
96-097098 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Ferreira, M.A.S.V. |
| 245 00 - TITLE STATEMENT |
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| Title |
Isolation of a species-specific mitochondrial DNA sequence for identification of Tilletia indica, the karnal bunt of wheat fungus |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Date of publication, distribution, etc. |
1996 |
| 340 ## - PHYSICAL MEDIUM |
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| Material base and configuration |
Printed |
| 500 ## - GENERAL NOTE |
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| General note |
references US (DNAL 448.3 Ap5) |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Mitochondrial DNA (mtDNA) from five isolates of Tilletia indica was isolated and digested with several restriction enzymes. A 2.3-kb EcoRI fragment was chosen, cloned, and shown to hybridize with total DNA restricted with EcoRI from T. indica and not from a morphologically similar smut fungus, Tilletia barclayana. The clone was partially sequenced, and primers were designed and tested under high-stringency conditions in PCR assays. The primer pair Ti1/Ti4 amplified a 2.3-kb fragment from total DNA of 17 T. indica isolates from India, Pakistan, and Mexico. DNA from 25 isolates of other smut fungi (T. barclayana, Tilletia foetida, Tilletia caries, Tilletia fusca, and Tilletia controversa) did not produce any bands, as detected by ethidium bromide-stained agarose gels and Southern hybridizations. The sensitivity of the assay was determined and increased by using a single nested primer in a second round of amplification, so that 1 pg of total mycelial DNA could be detected. The results indicated that the primers which originated from a cloned mtDNA sequence can be used to differentiate T. indica from other Tilletia species and have the potential to identify teliospores contaminating wheat seeds |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 595 ## - COLLECTION |
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| Collection |
AGRIS Collection |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Acids |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Basidiomycotina |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Cell structure |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Cytoplasm |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Cytoplasmic organelles |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Developmental stages |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Fungi |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1121 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Genetic engineering |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Genomes |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1131 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Gramineae |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Molecular hybridization |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Nucleic acids |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Nucleic compounds |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Organic acids |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Pathogenesis |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Plant developmental stages |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Plant diseases |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1206 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Plant genetics and breeding |
| Miscellaneous information |
NOT IN AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1208 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Protoplasm |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Tilletia |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Ustilaginales |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Triticum |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1295 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Castro, C., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hatziloukas, E., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Schaad, N.W., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Tooley, P.W., |
| Relator term |
coaut. |
| 773 0# - HOST ITEM ENTRY |
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| Title |
Applied and environmental microbiology (USA). (Jan 1996). v. 62(1) p. 87-93 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Article |