Knowledge Center Catalog

From repetitive DNA sequences to single-copy gene mapping in plant chromosomes by in situ hybridization (Record no. 11927)

MARC details
000 -LEADER
fixed length control field 01584nab a22003737a 4500
001 - CONTROL NUMBER
control field G28797
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170719154934.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title En
043 ## - GEOGRAPHIC AREA CODE
Geographic area code FR
072 #0 - SUBJECT CATEGORY CODE
Subject category code F30
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) REP-4725
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Mouras, A.
245 00 - TITLE STATEMENT
Title From repetitive DNA sequences to single-copy gene mapping in plant chromosomes by in situ hybridization
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 1989
340 ## - PHYSICAL MEDIUM
Material base and configuration Printed
500 ## - GENERAL NOTE
General note Illustrations, tables, references p. 168
520 ## - SUMMARY, ETC.
Summary, etc. Haploid leaf segments of Nicotiana plumbaginifolia were transformed with Agrobacterium rhizogenes and actively growing haploid root cultures established.Using protoplasts prepared from these cell populations, the single copy, 1.6 kb Adh (alcohol dehydrogenase) gene and repetitive ribosomal DNA sequences were tentatively mapped by in situ hybridization to chromosomes 1 or 5, and 1B, 6B and 5D respectively in metaphase preparations. The addition of sodium dodecylsulfate (SDS) and polyethylene glycol (PEG) to the probe mixture reduced background noise and allowed longer autoradiographic exposures (12-15 weeks)
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation Elsevier
595 ## - COLLECTION
Collection Reprints Collection
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Chromosomes
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element DNA
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Genetic maps
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Molecular Biology
9 (RLIN) 1183
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Protoplasts
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Horth, M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Jacobs, M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Negrutiu, I.,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Plant Physiology and Biochemistry
Note 609964
Related parts v. 27, no. 2, p. 161-168
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Holdings
Date last seen Total Checkouts Full call number Barcode Copy number Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
02/10/2015   REP-4725 609964 1 02/10/2015 Article Not Lost Dewey Decimal Classification     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 02/10/2015

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