High-throughput screening for photosynthetic efficiency and capacity in wheat
Silva-Pérez, V.
High-throughput screening for photosynthetic efficiency and capacity in wheat - 2012 - 1 page
Abstract only
A worldwide challenge is to increase yield potential in wheat to address global food demand. One way to improve yield is to increase crop biomass by improving photosynthesis, the main process whereby plants obtain the energy and CO2 required for biomass production. An important technique that helps to understand plant photosynthetic performance is the analysis of gas exchange with a portable photosynthesis system (for example LICOR LI-6400XT). Using a modelling approach and gas exchange measurements we can extract valuable information such as Rubisco amount and kinetics and electron transport rate to analyse photosynthetic efficiency and capacity in plants. However, this method is not very useful in high-throughput screening as measurements take considerable time. This research seeks to develop and validate new tools to screen for superior photosynthetic performance among a broad range of wheat germplasm. One approach has been to compare gas exchange measurements and destructive analysis with leaf spectral reflectance and absorbance, measured using SPAD and the ASD Field Spec 3 spectro-radiometer. Here we report on the potential of spectral reflectance to rapidly screen for variation in photosynthetic parameters in a set of 16 wheat genotypes grown in the glasshouse in Canberra, Australia. In addition, we are analysing the mechanisms underlying the variability in photosynthesis among these genotypes.
English
High-throughput screening for photosynthetic efficiency and capacity in wheat - 2012 - 1 page
Abstract only
A worldwide challenge is to increase yield potential in wheat to address global food demand. One way to improve yield is to increase crop biomass by improving photosynthesis, the main process whereby plants obtain the energy and CO2 required for biomass production. An important technique that helps to understand plant photosynthetic performance is the analysis of gas exchange with a portable photosynthesis system (for example LICOR LI-6400XT). Using a modelling approach and gas exchange measurements we can extract valuable information such as Rubisco amount and kinetics and electron transport rate to analyse photosynthetic efficiency and capacity in plants. However, this method is not very useful in high-throughput screening as measurements take considerable time. This research seeks to develop and validate new tools to screen for superior photosynthetic performance among a broad range of wheat germplasm. One approach has been to compare gas exchange measurements and destructive analysis with leaf spectral reflectance and absorbance, measured using SPAD and the ASD Field Spec 3 spectro-radiometer. Here we report on the potential of spectral reflectance to rapidly screen for variation in photosynthetic parameters in a set of 16 wheat genotypes grown in the glasshouse in Canberra, Australia. In addition, we are analysing the mechanisms underlying the variability in photosynthesis among these genotypes.
English