Mating type, cultural characteristic, and virulence of Bipolaris maydis in Thailand
Lapbanjob, S.
Mating type, cultural characteristic, and virulence of Bipolaris maydis in Thailand - Mexico, DF (Mexico) CIMMYT : 2004 - p. 441-450 - Printed
Survey and collecting of the fungus Bipolaris maydis, the causal agent of southern corn leaf blight (SCLB), were conducted in 26 maize growing areas throughout 19 provinces in 1999-2000 and were used for studying mating type, cultural characteristics, and virulence. The results suggested that SCLB has been found in almost all maize growing areas. Pure cultures of 118 isolates were collected and were assessed for morphological and cultural characteristics on potato dextrose agar (PDA). The results showed variation among 118 isolates in colony colour, uniformity of colony colour, aerial mycelium growth, and the zonation of colour and mycelium. Mycelial growth rate of 39 selected isolates of Bipolaris maydis was significantly different after growing the fungus on PDA. One hundred and eighteen isolates of Bipolaris maydis were investigated for mating type on modified Sachs' medium (in vitro). Fifty isolates were classified to mating type Ml-1 and 68 isolates were mating type Ml-2, accounted for 42.37 and 57.63%, respectively. Pairing of opposite mating type was done on maize plants (in vivo) and the result indicated that sexual reproduction has not been found. Twenty-one representative isolates were selected and evaluated for virulence on 22 inbred lines of maize. Significant difference was found among tested isolates and they showed no identical virulent patterns among them.
English
970-648-116-8
Bipolaris maydis
Cochliobolus heterostrophus
Infectious diseases
Maize
Plant diseases
Virulence
Yield factors
Hybrids
CIMMYT
Mating type, cultural characteristic, and virulence of Bipolaris maydis in Thailand - Mexico, DF (Mexico) CIMMYT : 2004 - p. 441-450 - Printed
Survey and collecting of the fungus Bipolaris maydis, the causal agent of southern corn leaf blight (SCLB), were conducted in 26 maize growing areas throughout 19 provinces in 1999-2000 and were used for studying mating type, cultural characteristics, and virulence. The results suggested that SCLB has been found in almost all maize growing areas. Pure cultures of 118 isolates were collected and were assessed for morphological and cultural characteristics on potato dextrose agar (PDA). The results showed variation among 118 isolates in colony colour, uniformity of colony colour, aerial mycelium growth, and the zonation of colour and mycelium. Mycelial growth rate of 39 selected isolates of Bipolaris maydis was significantly different after growing the fungus on PDA. One hundred and eighteen isolates of Bipolaris maydis were investigated for mating type on modified Sachs' medium (in vitro). Fifty isolates were classified to mating type Ml-1 and 68 isolates were mating type Ml-2, accounted for 42.37 and 57.63%, respectively. Pairing of opposite mating type was done on maize plants (in vivo) and the result indicated that sexual reproduction has not been found. Twenty-one representative isolates were selected and evaluated for virulence on 22 inbred lines of maize. Significant difference was found among tested isolates and they showed no identical virulent patterns among them.
English
970-648-116-8
Bipolaris maydis
Cochliobolus heterostrophus
Infectious diseases
Maize
Plant diseases
Virulence
Yield factors
Hybrids
CIMMYT