Expression of a synthetic porcine transgene increases lysine levels in maize kernels - Mexico, DF (Mexico) CIMMYT : 2003 - p. 167 - Printed
Cereal grains are major sources of nutrition for humans and other anima1s. The major cereals are defident in one or more of the essential amino acids such as lysine and tryptophan and, therefore, do not provide a well-balanced diet. These defidendes limit the nutritional value of the grain. Recent advances in genetic engineering and improvements in transformation technology have provided new opportunities to modify the nutritional composition of the maize grain. In an effort to elevate the amount of lysine in maize grain, a codon-adjusted version of a gene encoding the porcine milk protein a-lactalbumin was synthesized and used to transform maize. The goals in this study were: (1) to determine if the synthetic porcie a-La transgenes were integrated and functional in the maize genome; (2) to characterize the inheritance and expression patterns of the transgenes; and (3) to determine the leve1s of the a-La protein and the correlated changes in amino acid composition of transformed maize kemels.
631.53 / BOO