Cyto-swapping in maize by haploid induction with a cenh3 mutant
Bortiri, E.
Cyto-swapping in maize by haploid induction with a cenh3 mutant - United Kingdom : Nature Research, 2024.
Peer review
Maize mutants of the centromeric histone H3 (CENP-A/CENH3) gene can form haploids that inherit only chromosomes of the pollinating parent but the cytoplasm from the female parent. We developed CENH3 haploid inducers carrying a dominant anthocyanin colour marker for efficient haploid identification and harbouring cytoplasmic male sterile cytoplasm, a type of cytoplasm that results in male sterility useful for efficient hybrid seed production. The resulting cytoplasmic male sterility cyto-swapping method provides a faster and cheaper way to convert commercial lines to cytoplasmic male sterile compared to conventional trait introgression.
Text in English
2055-026X 055-0278 (Online)
https://doi.org/10.1038/s41477-024-01630-1
Maize
Haploidy
Gene editing
Plant growth
Cyto-swapping in maize by haploid induction with a cenh3 mutant - United Kingdom : Nature Research, 2024.
Peer review
Maize mutants of the centromeric histone H3 (CENP-A/CENH3) gene can form haploids that inherit only chromosomes of the pollinating parent but the cytoplasm from the female parent. We developed CENH3 haploid inducers carrying a dominant anthocyanin colour marker for efficient haploid identification and harbouring cytoplasmic male sterile cytoplasm, a type of cytoplasm that results in male sterility useful for efficient hybrid seed production. The resulting cytoplasmic male sterility cyto-swapping method provides a faster and cheaper way to convert commercial lines to cytoplasmic male sterile compared to conventional trait introgression.
Text in English
2055-026X 055-0278 (Online)
https://doi.org/10.1038/s41477-024-01630-1
Maize
Haploidy
Gene editing
Plant growth