Using precise genetic stocks to investigate the control of Stagonospora nodorum resistance in wheat
Ellerbrook, C.M.
Using precise genetic stocks to investigate the control of Stagonospora nodorum resistance in wheat - Mexico, DF (Mexico) CIMMYT : 1999 - Printed
A substitution series of ‘Synthetic 6x’ into ‘Chinese Spring’ had previously been studied to determine which of the 21 chromosomes of ‘Synthetic 6x’ conferred resistance to Stagonospora nodorum; from this single chromosome recombinant lines (SCRs) have been developed for substitution lines that improve the resistance of ‘Chinese Spring’ to the pathogen. The SCRs were screened for their response to infection. Marker assisted screening was also employed on the SCR populations; micro-satellite, RFLP and iso-electric focusing, coupled with conventional QTL analysis and morphological character scoring (vernalization response). As these markers had been previously mapped, it would be possible to pinpoint the resistance gene(s) and find a linked marker. Chromosome 5D had been shown to be the most effective against Stagonospora nodorum and is also known to carry the isozyme genes, Ibf-1 and Mdh-3, a range of micro-satellite markers, a sparse selection of RFLP markers, and a vernalization response gene (Vrn 3). Screening revealed that the resistance gene (Srb3) was located on the long arm between Ibf-1 and the RFLP probe Xpsr 912, but being 15.6 and 12.0 cM, respectively, away from Srb3, they could not be used as markers for resistance. SCRs have been developed for the remaining chromosomes, and screening continues to precisely map the resistance gene(s).
English
970-648-035-8
Chromosomes
Disease control
Genetic resistance
Plant diseases
Septoria
Stagonospora
Genetic resources
Triticum
CIMMYT
632.4 / GIN
Using precise genetic stocks to investigate the control of Stagonospora nodorum resistance in wheat - Mexico, DF (Mexico) CIMMYT : 1999 - Printed
A substitution series of ‘Synthetic 6x’ into ‘Chinese Spring’ had previously been studied to determine which of the 21 chromosomes of ‘Synthetic 6x’ conferred resistance to Stagonospora nodorum; from this single chromosome recombinant lines (SCRs) have been developed for substitution lines that improve the resistance of ‘Chinese Spring’ to the pathogen. The SCRs were screened for their response to infection. Marker assisted screening was also employed on the SCR populations; micro-satellite, RFLP and iso-electric focusing, coupled with conventional QTL analysis and morphological character scoring (vernalization response). As these markers had been previously mapped, it would be possible to pinpoint the resistance gene(s) and find a linked marker. Chromosome 5D had been shown to be the most effective against Stagonospora nodorum and is also known to carry the isozyme genes, Ibf-1 and Mdh-3, a range of micro-satellite markers, a sparse selection of RFLP markers, and a vernalization response gene (Vrn 3). Screening revealed that the resistance gene (Srb3) was located on the long arm between Ibf-1 and the RFLP probe Xpsr 912, but being 15.6 and 12.0 cM, respectively, away from Srb3, they could not be used as markers for resistance. SCRs have been developed for the remaining chromosomes, and screening continues to precisely map the resistance gene(s).
English
970-648-035-8
Chromosomes
Disease control
Genetic resistance
Plant diseases
Septoria
Stagonospora
Genetic resources
Triticum
CIMMYT
632.4 / GIN