Molecular characterization of the Glu-Ay gene from Triticum urartu for its potential use in quality wheat breeding
Gutierrez, M.V.
Molecular characterization of the Glu-Ay gene from Triticum urartu for its potential use in quality wheat breeding - 2011
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1479-2621
Triticum urartu Thum. ex Gandil. is a wild species identified as A-genome donor for polyploid wheats, which could be used as gene source for wheat breeding. The high-molecular weight glutenin subunits are endosperm storage proteins that are associated with bread-making quality. In T. urartu, these proteins are encoded by the Ax and Ay genes at the Glu-Au1 locus. The Ay gene of 17 Glu-Au1 allelic variants previously detected in this species has been analysed using PCR amplification and digestion of the PCR products with two endonucleases (DdeI and PstI). The combination of two restriction patterns has revealed variations between the active and inactive alleles, and within each type. This variation, especially that detected among the active alleles, could enlarge the high-quality genetic pool of modern wheat and be used for bread-making quality improvement in durum and common wheat.
English
1479-263X (Revista en electrónico) 1479-2621
https://doi.org/10.1017/S1479262111000220
Electrophoresis
glutenin
Quality breeding
Wild wheat
Genetic resources
Molecular characterization of the Glu-Ay gene from Triticum urartu for its potential use in quality wheat breeding - 2011
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1479-2621
Triticum urartu Thum. ex Gandil. is a wild species identified as A-genome donor for polyploid wheats, which could be used as gene source for wheat breeding. The high-molecular weight glutenin subunits are endosperm storage proteins that are associated with bread-making quality. In T. urartu, these proteins are encoded by the Ax and Ay genes at the Glu-Au1 locus. The Ay gene of 17 Glu-Au1 allelic variants previously detected in this species has been analysed using PCR amplification and digestion of the PCR products with two endonucleases (DdeI and PstI). The combination of two restriction patterns has revealed variations between the active and inactive alleles, and within each type. This variation, especially that detected among the active alleles, could enlarge the high-quality genetic pool of modern wheat and be used for bread-making quality improvement in durum and common wheat.
English
1479-263X (Revista en electrónico) 1479-2621
https://doi.org/10.1017/S1479262111000220
Electrophoresis
glutenin
Quality breeding
Wild wheat
Genetic resources