Barley yellow dwarf virus RNA has a protein covalently linked to its 5' terminus
Murphy, J.F.
Barley yellow dwarf virus RNA has a protein covalently linked to its 5' terminus - 1990 - Printed
10 ref
The RNA of an Illinois isolate of barley yellow dwarf virus (BYDV) transmitted specifically by Rhopalosiphum padi (L.) was examined to determine the status of its 5' terminus. Initial studies were to determine if the 5' terminus of BYDV RNA was blocked, i.e., contained any derivatization other than phosphate(s). BYDV RNA, satellite tobacco necrosis virus RNA (STNV RNA), positive control) and tobacco mosaic virus RNA (TMV RNA, negative control) were treated with calf intestinal phosphatase, then T4 polynucleotide kinase and [y-32P] ATP were added. The 5' terminus of STNV RNA was consistently labeled with 32P, however, neither BYDV RNA nor TMV RNA
English
968-6127-39
Acids
Aphididae
Aphidoidea
Arthropoda
Carbohydrates
Disease transmission
Enzymes
Glycosides
Hemiptera
Homoptera
Insecta
Luteoviruses
Nucleic acids
Nucleic compounds
Organic acids
Pathogenesis
Pests of plants
Plant diseases
Plant viruses
Proximate composition
Sternorrhyncha
Tobamoviruses
Viruses
91-013766
Barley yellow dwarf virus RNA has a protein covalently linked to its 5' terminus - 1990 - Printed
10 ref
The RNA of an Illinois isolate of barley yellow dwarf virus (BYDV) transmitted specifically by Rhopalosiphum padi (L.) was examined to determine the status of its 5' terminus. Initial studies were to determine if the 5' terminus of BYDV RNA was blocked, i.e., contained any derivatization other than phosphate(s). BYDV RNA, satellite tobacco necrosis virus RNA (STNV RNA), positive control) and tobacco mosaic virus RNA (TMV RNA, negative control) were treated with calf intestinal phosphatase, then T4 polynucleotide kinase and [y-32P] ATP were added. The 5' terminus of STNV RNA was consistently labeled with 32P, however, neither BYDV RNA nor TMV RNA
English
968-6127-39
Acids
Aphididae
Aphidoidea
Arthropoda
Carbohydrates
Disease transmission
Enzymes
Glycosides
Hemiptera
Homoptera
Insecta
Luteoviruses
Nucleic acids
Nucleic compounds
Organic acids
Pathogenesis
Pests of plants
Plant diseases
Plant viruses
Proximate composition
Sternorrhyncha
Tobamoviruses
Viruses
91-013766