Cowpea embryo rescue. 1. Influence of culture media composition on plant recovery from isolate immature embryos
Pellegrineschi, A.
Cowpea embryo rescue. 1. Influence of culture media composition on plant recovery from isolate immature embryos - Berlin (Germany) : Springer, 1997. - Printed
Factors responsible for successful rescue of immature embryos of cowpea [Vigna unguiculata (L.) Walp.] and V. vexillata (L.) and for in vitro embryo development were studied. A new basal medium for embryo development in vitro was formulated on the basis of the mineral composition of embryos. Sucrose, fructose and glucose were compared as carbohydrate sources. The highest frequency of embryos developing into plants was obtained with sucrose. Adding casein hydrolysate to the medium increased plant recovery by 30%. Among the plant growth factors used, cytokinins, zeatin, 6-benzylaminopurine and kinetin were the most effective in promoting embryo maturation and development. A method that can routinely ensure high plant recovery from cultured immature cowpea embryos is proposed.
Text in English
0721-7714 1432-203X (Online)
https://doi.org/10.1007/s002990050366
Carbohydrate content
Cowpeas
Crop yield
Embryonic development
Fields
Fructose
Glucose
Grain
Hydrolases
In vitro culture
Mineral content
Sucrose
Vigna unguiculata
Zeatin
Cowpea embryo rescue. 1. Influence of culture media composition on plant recovery from isolate immature embryos - Berlin (Germany) : Springer, 1997. - Printed
Factors responsible for successful rescue of immature embryos of cowpea [Vigna unguiculata (L.) Walp.] and V. vexillata (L.) and for in vitro embryo development were studied. A new basal medium for embryo development in vitro was formulated on the basis of the mineral composition of embryos. Sucrose, fructose and glucose were compared as carbohydrate sources. The highest frequency of embryos developing into plants was obtained with sucrose. Adding casein hydrolysate to the medium increased plant recovery by 30%. Among the plant growth factors used, cytokinins, zeatin, 6-benzylaminopurine and kinetin were the most effective in promoting embryo maturation and development. A method that can routinely ensure high plant recovery from cultured immature cowpea embryos is proposed.
Text in English
0721-7714 1432-203X (Online)
https://doi.org/10.1007/s002990050366
Carbohydrate content
Cowpeas
Crop yield
Embryonic development
Fields
Fructose
Glucose
Grain
Hydrolases
In vitro culture
Mineral content
Sucrose
Vigna unguiculata
Zeatin