Characterization of high-and low-molecular weight glutenin subunits associated to the D genome of aegilops tauschii in a collection of synthetic hexaploid wheats
Pflüger, L.A.
Characterization of high-and low-molecular weight glutenin subunits associated to the D genome of aegilops tauschii in a collection of synthetic hexaploid wheats - Berlin (Germany) : Springer, 2001. - Printed
Peer-review: Yes - Open Access: Yes*http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752 Peer reivew
Synthetic hexaploid wheats (2n=6x=42, AA-BBDD) involving genomes from Triticum turgidum (2n=4x=28, AABB) and Aegilops tauschii (2n=2x=14, DD) have been produced as a means for introducing desirable characteristics into bread wheat. In the present work we describe the genetic variability present at the Glu-D11 and Glu-D13 loci, encoding high- (HMW) and low-molecular-weight (LMW) glutenin subunits respectively, derived from Ae. tauschii, using electrophoretic and chromatographic methods, in a collection of synthetic hexaploid wheats. A wide variation both in mobility and surface hydrophobicity of HMW glutenin subunits was observed between different accessions of Ae. tauschii used in the production of the synthetic hexaploids. A combination of electrophoretic and chromatographic methods improves the identification of HMW glutenin subunits; in fact subunits with identical apparent mobility were revealed to have a different surface hydrophobicity by reversed-phase high performance liquid chromatography. None of the DxSt subunits present in Ae. tauschii showed the presence of the extra cysteine residue found in the HMW glutenin subunit DxS of Triticum aestivum, as revealed by selective amplification with polymerase chain reaction (PCR). The wide variability and the high number of subunits encoded by the Glu-DI3, locus suggests that Ae. tauschii may be a rich source for enhancing the genetic variability of glutenin subunits in bread wheat and improving bread-making properties.
Text in English
0040-5752 1432-2242 (Online)
https://doi.org/10.1007/s001220100704
Aegilops tauschii
Hexaploidy
Electrophoresis
Wheat
Characterization of high-and low-molecular weight glutenin subunits associated to the D genome of aegilops tauschii in a collection of synthetic hexaploid wheats - Berlin (Germany) : Springer, 2001. - Printed
Peer-review: Yes - Open Access: Yes*http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752 Peer reivew
Synthetic hexaploid wheats (2n=6x=42, AA-BBDD) involving genomes from Triticum turgidum (2n=4x=28, AABB) and Aegilops tauschii (2n=2x=14, DD) have been produced as a means for introducing desirable characteristics into bread wheat. In the present work we describe the genetic variability present at the Glu-D11 and Glu-D13 loci, encoding high- (HMW) and low-molecular-weight (LMW) glutenin subunits respectively, derived from Ae. tauschii, using electrophoretic and chromatographic methods, in a collection of synthetic hexaploid wheats. A wide variation both in mobility and surface hydrophobicity of HMW glutenin subunits was observed between different accessions of Ae. tauschii used in the production of the synthetic hexaploids. A combination of electrophoretic and chromatographic methods improves the identification of HMW glutenin subunits; in fact subunits with identical apparent mobility were revealed to have a different surface hydrophobicity by reversed-phase high performance liquid chromatography. None of the DxSt subunits present in Ae. tauschii showed the presence of the extra cysteine residue found in the HMW glutenin subunit DxS of Triticum aestivum, as revealed by selective amplification with polymerase chain reaction (PCR). The wide variability and the high number of subunits encoded by the Glu-DI3, locus suggests that Ae. tauschii may be a rich source for enhancing the genetic variability of glutenin subunits in bread wheat and improving bread-making properties.
Text in English
0040-5752 1432-2242 (Online)
https://doi.org/10.1007/s001220100704
Aegilops tauschii
Hexaploidy
Electrophoresis
Wheat