Production of polyploid wheat by nitrous oxide
Kihara, H.
Production of polyploid wheat by nitrous oxide - Japan : The Japan Academy, 1960. - Printed
Free Access
N2O treatment proved to be an efficient means of producing polyploids in wheat. Spikes of Triticum dicoccum Khapli which were pollinated 24 hours before treatment were exposed to nitrous oxide at the pressure of 3 or 6 atmospheres for each of the 5, 10, and 15 hour periods and then cultured in an artificial medium. Treatments for 5 hours did not induce any aberration of chromosome number. Treatments at both 3 and 6 atm, pressures for 10 or 15 hours produced tetraploids and aneuploids in 70 per cent or more of plants obtained from treated flowers. A great majority of the aneuploids were hypotetraploids. The best success was obtained by the treatment at 6 atm, pressure for 15 hours, by which 98 per cent of treated florets gave polyploids and aneuploids. The advantage of N2O treatment over colchicine treatment as a means of producing polyploids was discussed.
Text in English
0021-4280
https://doi.org/10.2183/pjab1945.36.658
Polyploidy
Wheat
Nitrous oxide
Experimentation
Production of polyploid wheat by nitrous oxide - Japan : The Japan Academy, 1960. - Printed
Free Access
N2O treatment proved to be an efficient means of producing polyploids in wheat. Spikes of Triticum dicoccum Khapli which were pollinated 24 hours before treatment were exposed to nitrous oxide at the pressure of 3 or 6 atmospheres for each of the 5, 10, and 15 hour periods and then cultured in an artificial medium. Treatments for 5 hours did not induce any aberration of chromosome number. Treatments at both 3 and 6 atm, pressures for 10 or 15 hours produced tetraploids and aneuploids in 70 per cent or more of plants obtained from treated flowers. A great majority of the aneuploids were hypotetraploids. The best success was obtained by the treatment at 6 atm, pressure for 15 hours, by which 98 per cent of treated florets gave polyploids and aneuploids. The advantage of N2O treatment over colchicine treatment as a means of producing polyploids was discussed.
Text in English
0021-4280
https://doi.org/10.2183/pjab1945.36.658
Polyploidy
Wheat
Nitrous oxide
Experimentation