Exponential amplification of recombinant-RNA hybridization probes
Lizardi, P.M.
Exponential amplification of recombinant-RNA hybridization probes - 1988 - Printed
Illustrations, graphs, references p. 1202
Recombinant RNA molecules were synthesized that function both as hybridization probes and as templates for exponential amplification by Qbeta replicase. Each recombinant consists of a sequence specific for Plasmodium falciparum, embedded within the sequence of MDV-1 RNA, which is a natural template for Qbeta replicase. The probe sequence was inserted within a hairpin loop that occurs on the exterior of MDV-1 RNA. The recombinant RNAs hybridize specifically to complementary DNA, despite topological constraints on the probe domain, are replicated at the same rate as MDV-1 RNA, despite their additional length, and are able to serve as templates for the
English
Biotechnology
Plasmodium
RNA
Exponential amplification of recombinant-RNA hybridization probes - 1988 - Printed
Illustrations, graphs, references p. 1202
Recombinant RNA molecules were synthesized that function both as hybridization probes and as templates for exponential amplification by Qbeta replicase. Each recombinant consists of a sequence specific for Plasmodium falciparum, embedded within the sequence of MDV-1 RNA, which is a natural template for Qbeta replicase. The probe sequence was inserted within a hairpin loop that occurs on the exterior of MDV-1 RNA. The recombinant RNAs hybridize specifically to complementary DNA, despite topological constraints on the probe domain, are replicated at the same rate as MDV-1 RNA, despite their additional length, and are able to serve as templates for the
English
Biotechnology
Plasmodium
RNA