Plant regeneration from mesocotyl callus of Hordeum vulgare L
Jelaska, S.
Plant regeneration from mesocotyl callus of Hordeum vulgare L - 1984 - Printed
Callus tissue was induced on mesocotyl explants of 7 day old seedlings on Murashige & Skoog (MS) medium supplemented with high concentrations of 2,4 D or 2,4,5 T. Two morphologically different tissue cultures were maintained in vitro for a long time: a callus tissue without organogenesis and a culture with high rhizogenic ability. Shoots and plantlets were regenerated when the callus was transferred to a medium supplemented with 3 æM TIBA. The largest number of plants obtained per mesocotyl was 40 and some cultures produced shoots even after 9 subcultures (about 14 months). Plantlets rooted well on MS medium with 5.7 æM IAA and survived
English
Barley
Cereals
Hordeum vulgare
In vitro experimentation
Isolation techniques
Isolation techniques
Regeneration
Tissue culture
Genotypes
Plant regeneration from mesocotyl callus of Hordeum vulgare L - 1984 - Printed
Callus tissue was induced on mesocotyl explants of 7 day old seedlings on Murashige & Skoog (MS) medium supplemented with high concentrations of 2,4 D or 2,4,5 T. Two morphologically different tissue cultures were maintained in vitro for a long time: a callus tissue without organogenesis and a culture with high rhizogenic ability. Shoots and plantlets were regenerated when the callus was transferred to a medium supplemented with 3 æM TIBA. The largest number of plants obtained per mesocotyl was 40 and some cultures produced shoots even after 9 subcultures (about 14 months). Plantlets rooted well on MS medium with 5.7 æM IAA and survived
English
Barley
Cereals
Hordeum vulgare
In vitro experimentation
Isolation techniques
Isolation techniques
Regeneration
Tissue culture
Genotypes