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A North-South partnership identifies genetic biodiversity for durable rust resistance in African wheat genotypes

by Prins, R; Agenbag, G.M; Boyd, L.A; Pretorius, Z.A; Dreisigacker, S.
Material type: materialTypeLabelBookPublisher: 2013Description: p. 4.Summary: Since 1996 Prins, Boyd and Pretorius have characterised stripe rust resistances in South African wheat varieties. In 2008 the team was awarded a SARID grant (UK: BBSRC-DfID initiative), expanding their research to include genetic characterisation of stem rust resistance, to build DNA marker capacity, and to train a PhD student. The SARID project had three goals: (1) to fine map stripe rust adult plant resistance (APR) QTL on chromosomes 2B and 4A identified in the South African variety Kariega, (2) to genetically analyse stripe rust APR derived from the European variety Cappelle-Desprez, and (3) to assess genetic diversity for stem rust and stripe rust resistances within the John Innes Center African wheat collection. The fine mapping of the Kariega QTL resulted in the establishment of new marker technology within the South African team, developing markers for single nucleotide polymorphisms (SNPs) within wheat genes identified through comparative cereal genomic analyses. These SNP markers defined each QTL to a smaller genetic interval, proving highly valuable for marker assisted selection (MAS), with one SNP marker being diagnostic for the valuable 4A QTL. One major and 3 minor QTL for stripe rust APR were identified in the Cappelle-Desprez-derived line Yr16DH70, providing a valuable resource for wheat stripe rust resistance breeding within South Africa. These QTL and associated DNA tools are now being used by South African wheat breeders. To identify new sources of stripe rust and stem rust APR a genetic association analysis (GAA) was undertaken in a collection of African wheat lines. This project established a new and highly successful collaboration between Prins and Dreisigacker (CIMMYT) and introduced GAA into wheat research in South Africa. The stem rust screens were particularly valuable, identifying seedling and APR effective against the Ug99 race group. Two wheat lines with APR to stem rust were crossed with a susceptible parent and doubled haploid populations were developed to validate the GAA analysis.Collection: CIMMYT Staff Publications Collection
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Procedings
CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Staff Publications Collection CIS-7271 (Browse shelf) Available
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Abstract only

Since 1996 Prins, Boyd and Pretorius have characterised stripe rust resistances in South African wheat varieties. In 2008 the team was awarded a SARID grant (UK: BBSRC-DfID initiative), expanding their research to include genetic characterisation of stem rust resistance, to build DNA marker capacity, and to train a PhD student. The SARID project had three goals: (1) to fine map stripe rust adult plant resistance (APR) QTL on chromosomes 2B and 4A identified in the South African variety Kariega, (2) to genetically analyse stripe rust APR derived from the European variety Cappelle-Desprez, and (3) to assess genetic diversity for stem rust and stripe rust resistances within the John Innes Center African wheat collection. The fine mapping of the Kariega QTL resulted in the establishment of new marker technology within the South African team, developing markers for single nucleotide polymorphisms (SNPs) within wheat genes identified through comparative cereal genomic analyses. These SNP markers defined each QTL to a smaller genetic interval, proving highly valuable for marker assisted selection (MAS), with one SNP marker being diagnostic for the valuable 4A QTL. One major and 3 minor QTL for stripe rust APR were identified in the Cappelle-Desprez-derived line Yr16DH70, providing a valuable resource for wheat stripe rust resistance breeding within South Africa. These QTL and associated DNA tools are now being used by South African wheat breeders. To identify new sources of stripe rust and stem rust APR a genetic association analysis (GAA) was undertaken in a collection of African wheat lines. This project established a new and highly successful collaboration between Prins and Dreisigacker (CIMMYT) and introduced GAA into wheat research in South Africa. The stem rust screens were particularly valuable, identifying seedling and APR effective against the Ug99 race group. Two wheat lines with APR to stem rust were crossed with a susceptible parent and doubled haploid populations were developed to validate the GAA analysis.

Global Wheat Program

English

Lucia Segura

INT2692

CIMMYT Staff Publications Collection

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